Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Locations
Orders Processing,
Shipping & Receiving,
Warehouse
2 Shaker Rd Suites
B001/B101
Shirley, MA 01464
Production Lab
Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036
Telephone Numbers
Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-C209481-500 | 500 µg (1 mg/ml) | $472 |
Polyclonal Goat Anti‑Mouse IgG2a Secondary Antibody (Atto 425, IF, WB) LS‑C209481
Polyclonal Goat Anti‑Mouse IgG2a Secondary Antibody (Atto 425, IF, WB) LS‑C209481
Antibody:
Mouse IgG2a Goat anti-Mouse Polyclonal (Atto 425) Antibody
Application:
IF, WB, Flo, FLISA
Reactivity:
Mouse
Format:
Atto 425, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Mouse IgG2a Goat anti-Mouse Polyclonal (Atto 425) Antibody
Application:
IF, WB, Flo, FLISA
Reactivity:
Mouse
Format:
Atto 425, Unmodified
Specifications
Description
Mouse IgG2a antibody LS-C209481 is an Atto 425-conjugated goat polyclonal antibody. Validated for FLISA, Flow, IF and WB.
Host
Goat
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Atto 425
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Mouse IgG2a heavy chain.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Applications
- Immunofluorescence (1:5000)
- Western blot (1:4000 - 1:20000)
- Flow Cytometry (1:500 - 1:2500)
- Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
Anti-Mouse IgG2a secondary antibody is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG2a antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Mouse
Applications:
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Mouse
Applications:
ELISA
Species:
Mouse
Applications:
IHC, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
Date Requested: 4/27/2024
