Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse (tested or 100% immunogen sequence identity)
IHC (1:500 - 1:3000)
Western blot (1:1000)
Specificity and Use
Mouse IgG1 antibody was raised against mouse IgG1 heavy chain.
Recognizes mouse IgG1. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. Specificity was confirmed by ELISA. Typically less than 1% cross-reactivity was observed against other mouse heavy chain isotypes.
Suitable for use in ELISA, Immunohistochemistry, Western Blot and Immunomicroscopy. Also suitable for other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. ELISA: 1:25000. Immunohistochemistry: 1:500-1:3000. Western Blot: 1:1000-1-5000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from PBS, pH 7.2, 10 mg/ml BSA (IgG and Protease free), 0.01% sodium azide
1,000 µl Sterile ddH2O
Store at -20°C lyophilized. Reconstitute with sterile 40-50% glycerol and PBS. Aliquot and store at -20°C.
For research use only.
About Mouse IgG1
IgG1 is the most abundant subclass of IgG, and many mouse monoclonal antibodies are IgG1.