(tested or 100% immunogen sequence identity)
- Western blot
- ELISA (1:1600)
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Human IgG Fc antibody was raised against human IgG F(c) fragment.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, human IgG, human IgG F(c) and human serum. No reaction was observed against human IgG F (ab')2.
Suitable for use in Western Blot (Western or Dot Blot), ELISA and Immunohistochemistry. ELISA: 1:1600 to 1:16000 using pNPP, p-nitrophenyl phosphate, as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
TBS, 1 mM magnesium chloride, 0.1 mM zinc chloride, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide, 50% glycerol.
Store at 4°C. Do not freeze.
For research use only.
This antibody carries the LSBio 100% Guarantee.