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Rabbit Anti‑Hemagglutinin / HA Tag Secondary Antibody LS‑C154224

Catalog Number Size Price
LS-C154224-100 100 µg (1 mg/ml) $335 
Anti-HA Epitope Tag Antibody - Immunohistochemistry. Affinity Purified anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by IHC on formalin fixed paraffin embedded tissue. Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 micron were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2000 and reacted at 4° C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayers hematoxylin. Personal Communication, Behzad Yeganeh, U. Manitoba, Winnipeg, Canada.
Anti-HA Epitope Tag Antibody - Western Blot. Anti-HA epitope tag polyclonal antibody detects HA-tagged recombinant proteins by western blot. Polyclonal Rabbit anti-HA epitope tag, at a 1:2000 dilution, was used to detect 1.0 ug of 12-Epitope Tag Protein Marker Lysate (MB- containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Anti-HA Epitope Tag Antibody - Immunohistochemistry. Affinity Purified anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by IHC on formalin fixed paraffin embedded tissue. Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 micron were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2000 and reacted at 4° C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayers hematoxylin. Personal Communication, Behzad Yeganeh, U. Manitoba, Winnipeg, Canada.
Anti-HA Epitope Tag Antibody - Western Blot. Anti-HA epitope tag polyclonal antibody detects HA-tagged recombinant proteins by western blot. Polyclonal Rabbit anti-HA epitope tag, at a 1:2000 dilution, was used to detect 1.0 ug of 12-Epitope Tag Protein Marker Lysate (MB- containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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Rabbit Anti‑Hemagglutinin / HA Tag Secondary Antibody LS‑C154224

Antibody:
Rabbit Polyclonal to Hemagglutinin / HA Tag
Application:
IHC, IHC-P, WB, IP, ELISA, ChrIP
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335 
LS-C154224-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Hemagglutinin / HA Tag
Application:
IHC, IHC-P, WB, IP, ELISA, ChrIP
Format:
Unconjugated, Unmodified

Specifications

Description
HA Tag antibody LS-C154224 is an unconjugated rabbit polyclonal antibody. Validated for ChrIP, ELISA, IHC, IP and WB.
Target
Hemagglutinin / HA Tag
Host
Rabbit
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500 - 1:2000)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation
  • ELISA
  • Chromatin Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the C-terminus end to facilitate coupling.
Epitope
C-Terminus
Specificity
This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Usage
Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Product
Species:
Applications: IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry
Detection of HA-tagged fusion protein in 200, 100, and 50ng of E. coli cell lysate
Species:
Applications: ICC, Western blot, Immunoprecipitation, ELISA
Product
Species: Virus
Applications: IHC - Paraffin, Western blot, ELISA
Reactivity: Human
Range: 0.156-10 ng/ml

Requested From: United States
Date Requested: 8/18/2019
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