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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Hemagglutinin / HA Tag

HA Tag / Hemagglutinin Tag

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The amino acid sequence is: YPYDVPDYA

Hemagglutinin / HA Tag Target Details

Target Name: HA Tag / Hemagglutinin Tag
Target Abbreviation: HA Tag

Publications (4)

1
Immunization with reverse-genetics-produced H5N1 influenza vaccine protects ferrets against homologous and heterologous challenge. Govorkova EA, Webby RJ, Humberd J, Seiler JP, Webster RG. The Journal of infectious diseases. 2006 194:159-67. [PubMed:16779721]
2
Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice. Hanson BJ, Boon AC, Lim AP, Webb A, Ooi EE, Webby RJ. Respiratory research. 2006 7:126. [PubMed:17040574] [PMC:PMC1626464]
3
Amino acid residues in the fusion peptide pocket regulate the pH of activation of the H5N1 influenza virus hemagglutinin protein. Reed ML, Yen HL, DuBois RM, Bridges OA, Salomon R, Webster RG, Russell CJ. Journal of virology. 2009 83:3568-80. [PubMed:19193808] [PMC:PMC2663236]
4
FOXO3 modulates endothelial gene expression and function by classical and alternative mechanisms. Czymai T, Viemann D, Sticht C, Molema G, Goebeler M, Schmidt M. The Journal of biological chemistry. 2010 285:10163-78. [PubMed:20123982] [PMC:PMC2856222] Related Antibodies: LS-C157795.
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Filtered by: None
Target
Hemagglutinin / HA Tag (152)
Reactivity
Human (4)
Mouse (6)
All species (8)
Influenza Virus (6)
Rabbit (2)
Virus (5)
Application
IHC (19)
IHC-Fr (1)
IHC-P (3)
WB (136)
Flo (9)
ChrIP (2)
DB (1)
ELISA (87)
ICC (17)
IF (23)
IP (51)
Micro (2)
Host
rabbit (60)
mouse (72)
goat (7)
chicken (12)
sheep (1)
Isotype
Ig (1)
IgG (55)
IgG1 (9)
IgG1,k (9)
IgG2a (8)
IgG2a,k (1)
IgG2b (13)
IgG3 (11)
IgG3,k (1)
IgY (5)
Clonality
monoclonal mc (71)
polyclonal pc (81)
Clone
12CA5 (6)
16B12 (5)
1809CT823.37.18 (1)
1B10 (1)
2C1 (1)
2K6 (1)
4G3 (1)
4G8 (1)
5D8 (1)
5E11D8 (3)
6G2A9 (1)
7D3 (1)
9D5 (1)
AT5E7 (1)
HA.C5 (2)
HA.C5 / C10 (1)
Mix (1)
PAT5E7AT (1)
T80 (1)
Format
Agarose Conjugated (1)
AP Conjugated (9)
APC Conjugated (6)
Azide-free (1)
Biotin (NHS-LC) Conjugated (1)
Biotin Conjugated (12)
FITC Conjugated (12)
HiLyte Fluor 488 Conjugated (1)
HiLyte Fluor 555 Conjugated (1)
HiLyte Fluor 647 Conjugated (1)
HRP Conjugated (19)
PE Conjugated (7)
Preservative Free (1)
RPE Conjugated (1)
Unconjugated (81)
Epitope
C-Terminus (13)
Internal (2)
aa114-122 (2)
N-Terminus (1)
aa98-106 (1)
Publications
No (151)
Yes (1)
Showing 1-25 of 152 results

Hemagglutinin / HA Tag Antibody (C-Terminus) LS-C154224
Rabbit Polyclonal (IgG)
ChrIP, ELISA, IHC, IHC-P, IP, WB
Unconjugated
100µg 1mg/ml  $335.00
Anti-HA Epitope Tag Antibody - Immunohistochemistry. Affinity Purified anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by IHC on formalin fixed paraffin embedded tissue. Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 micron were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2000 and reacted at 4° C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayers hematoxylin. Personal Communication, Behzad Yeganeh, U. Manitoba, Winnipeg, Canada.
Anti-HA Epitope Tag Antibody - Western Blot. Anti-HA epitope tag polyclonal antibody detects HA-tagged recombinant proteins by western blot. Polyclonal Rabbit anti-HA epitope tag, at a 1:2000 dilution, was used to detect 1.0 ug of 12-Epitope Tag Protein Marker Lysate (MB- containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Hemagglutinin / HA Tag Antibody (C-Terminus) LS-C56781
Rabbit Polyclonal (IgG) to Virus Hemagglutinin / HA Tag
Virus
ELISA, IHC-P, WB
Unconjugated
50µg 0.5mg/ml  $315.00

Hemagglutinin / HA Tag Antibody LS-C83281
Rabbit Polyclonal
ELISA, ICC, IP, WB
Unconjugated
100µg  $355.00; 1mg  $1,140.00
Detection of HA-tagged fusion protein in 200, 100, and 50ng of E. coli cell lysate

Hemagglutinin / HA Tag Antibody (C-Terminus) LS-C50863
Mouse Monoclonal (IgG1)
Flo, ICC, IF, IHC, WB
Unconjugated
100µl  $530.00

Hemagglutinin / HA Tag Antibody (C-Terminus) LS-C50851
Chicken Polyclonal (IgY)
ELISA, IHC, WB
Unconjugated
1ml  $575.00

Hemagglutinin / HA Tag Antibody LS-C136213
Sheep Polyclonal (IgG)
IHC, WB
Unconjugated
500µg  $325.00

Hemagglutinin / HA Tag Antibody (Biotin) LS-C520093
Mouse Monoclonal (IgG2b)
ELISA, WB
Biotin Conjugated
200µl  $740.00

Hemagglutinin / HA Tag Antibody (clone 12CA5, PE) LS-C259980
Mouse Monoclonal [clone 12CA5] (IgG2b)
ELISA, WB
PE Conjugated
200µl 0.55mg/ml  $740.00

Hemagglutinin / HA Tag Antibody LS-C413451
Mouse Monoclonal
ICC, IF, IP, WB
Unconjugated
100µl 1mg/ml  $275.00
Immunofluorescent analysis of HA-tag staining in 293T cells transfected with a HA-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of over-expressed HA-tagged protein in 293T cell lysate.

Hemagglutinin / HA Tag Antibody LS-C157795
Publications (1)
Publications (1)
Mouse Monoclonal (IgG2b)
WB
Unconjugated
200µl  $325.00
HA-tagged recombinant protein was expressed in COS7 cells. 15 ug of cell lysate was loaded onto a gel to test the HA tag antibody.
Western blot of anti-HA Tag Antibody in 12tag recombinant protein. 12tag recombinant protein was detected using the purified antibody.

Hemagglutinin / HA Tag Antibody (HiLyte Fluor 488) LS-C742056
Mouse Monoclonal (IgG1,k)
Flo, IF
HiLyte Fluor 488 Conjugated
100µg  $455.00
Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) or HA-tagged protein transfected CHO cells (b) using THE TM HA Tag Antibody [iFluor 488], mAb, Mouse.
Flow cytometric analysis of non-transfected CHO cells (Black) or HA-tagged protein transfected CHO cells (Red) using THE TM HA Tag Antibody [iFluor 488], mAb, Mouse.

Hemagglutinin / HA Tag Antibody (aa114-122, FITC) LS-C50858
Goat Polyclonal (IgG)
ELISA
FITC Conjugated
100µl  $590.00

Hemagglutinin / HA Tag Antibody LS-C157796
Rabbit Polyclonal
ChrIP, IF, WB
Unconjugated
200µl  $325.00
GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007))
Western blot of lysate from 12 tag recombinant protein cell line, using Tag-HA. 2x Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Hemagglutinin / HA Tag Antibody LS-C123731
Mouse Monoclonal (IgG1)
IHC, IP, WB
Unconjugated
100µg  $495.00

Hemagglutinin / HA Tag Antibody (C-Terminus, Biotin) LS-C50923
Rabbit Polyclonal
ELISA, IHC, WB
Biotin Conjugated
100µg  $740.00

Hemagglutinin / HA Tag Antibody (Biotin) LS-C526494
Mouse Monoclonal (IgG3)
ICC, IP, WB
Biotin Conjugated
100µl 0.85mg/ml  $980.00

Hemagglutinin / HA Tag Antibody LS-C51072
Goat Polyclonal (IgG)
ELISA, WB
Unconjugated
40µg  $245.00; 100µg 0.5mg/ml  $325.00
Western blot of HA-tagged fusion protein using HA-tag Antibody, pAb, Goat (HA-tag Antibody, pAb, Goat, 1 ug/ml) The signal was developed with Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit Predicted Size: 52 kD Observed Size: 52 kD
Western blot of HA tagged fusion protein using 1 ug/ml Goat Anti-HA-tag Polyclonal Antibody HA-tag Antibody, pAb, Goat Lane 1-8: N-terminal or C-terminal HA tagged fusion protein cell lysate (~31 kD) Secondary antibody: Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody The signal was developed with LumiSensor HRP Substrate Kit

Hemagglutinin / HA Tag Antibody LS-C114296
Mouse Monoclonal (IgG3)
IP, WB
Unconjugated
100µl 1mg/ml  $295.00
Western blot Image

Hemagglutinin / HA Tag Antibody LS-C129638
Rabbit Polyclonal
IP, WB
Unconjugated
50µg  $425.00

Hemagglutinin / HA Tag Antibody (clone 5E11D8, HRP) LS-C203612
Mouse Monoclonal [clone 5E11D8] (IgG1,k)
ELISA, WB
HRP Conjugated
100µg  $415.00
Western blot of Multiple Tag using HA Tag Antibody [HRP], mAb, Mouse (0. 5 ug/ml). . Predicted Size: 52 kD. Observed Size: 52 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Hemagglutinin / HA Tag Antibody (Biotin) LS-C130006
Rabbit Monoclonal (IgG)
Flo, WB
Biotin Conjugated
100µl  $590.00

Hemagglutinin / HA Tag Antibody LS-C204870
Mouse Monoclonal (IgG2b)
ELISA
Unconjugated
0.5mg  $335.00; 1mg  $445.00

Hemagglutinin / HA Tag Antibody LS-C483522
Mouse Monoclonal to Influenza Virus Hemagglutinin / HA Tag
Influenza Virus
ELISA, Flo, ICC, IF, IP, WB
Unconjugated
50µl  $295.00; 100µl  $365.00; 500µl  $605.00; 1ml  $905.00

Hemagglutinin / HA Tag Antibody (C-Terminus, HRP) LS-C67394
Goat Polyclonal (IgG)
ELISA, WB
HRP Conjugated
40µg  $405.00; 100µg  $515.00

Hemagglutinin / HA Tag Antibody (HRP) LS-C51073
Goat Polyclonal (IgG)
ELISA, WB
HRP Conjugated
40µg  $245.00; 100µg 0.5mg/ml  $325.00
Western blot of HA-tagged fusion protein using HA-tag Antibody [HRP], pAb, Goat (HA-tag Antibody [HRP], pAb, Goat, 1 ug/ml) The signal was developed with LumiSensor HRP Substrate Kit. Predicted Size: 52 kD Observed Size: 52 kD This image was taken for the unconjugated form of this product. Other forms have not been tested.
Showing 1-25 of 152 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).