Human PXN / Paxillin
Human, Mouse, Rat, Bovine, Chicken
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
GST fusion protein containing full-length human paxillin.
Recognizes human Paxillin at 68kD and a band around 50kD, which is most likely a degradation product. Species cross-reactivity: Human, mouse, rat, chicken and bovine. Other species not tested.
- ICC (5 - 10 µg/ml)
- Western blot (0.5 - 2 µg/ml)
Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Western Blot: 0.5-2 ug/ml detects paxillin in RIPA lysates from 3T3 fibroblasts using 20 ug per lane for minigels. Immunoprecipitation: 4 ug per 500 ug of cell lysate. Immunocytochemistry:5-10 ug/ml (gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7% paraformaldehyde for 5 minutes).
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.