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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PSME1 Antibody (aa1‑50) LS‑C290507
PSME1 antibody LS-C290507 is an unconjugated rabbit polyclonal antibody to human PSME1. Validated for IP and WB.
Catalog
Size
Price
LS-C290507-100
100 µl (1 mg/ml)
Unavailable

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Product Description

PSME1 antibody LS-C290507 is an unconjugated rabbit polyclonal antibody to human PSME1. Validated for IP and WB.
About PSME1
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Q06323 NM_006263 NP_006254.1

PSME1 Antibody, 29-kD MCP activator subunit Antibody, IFI5111 Antibody, IGUP I-5111 Antibody, REG-alpha Antibody, REGalpha Antibody, PA28A Antibody, Interferon-gamma IEF SSP 5111 Antibody, PA28alpha Antibody, Proteasome activator subunit-1 Antibody


Specifications

Target
Human PSME1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:5000)
  • Immunoprecipitation (2 - 10 µg/mg lysate)
Epitope
aa1-50
Specificity
Region between residue 1 and 50 of human Proteasome (Prosome, Macropain) Activator Subunit 1 (PA28 alpha) using the numbering given in entry NP_006254.1 (GeneID 5720).
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

Detection of Human PSME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for IP at 6 ug/mg lysate. PSME1 was also immunoprecipitated by rabbit anti-PSME1 antibody BL13937. For blotting immunoprecipitated PSME1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human PSME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for IP at 6 ug/mg lysate. PSME1 was also immunoprecipitated by rabbit anti-PSME1 antibody BL13937. For blotting immunoprecipitated PSME1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western blot

Detection of Human PSME1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human PSME1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western blot

Detection of Human PSME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for IP at 6 ug/mg lysate. PSME1 was also immunoprecipitated by rabbit anti-PSME1 antibody BL13937. For blotting immunoprecipitated PSME1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human PSME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for IP at 6 ug/mg lysate. PSME1 was also immunoprecipitated by rabbit anti-PSME1 antibody BL13937. For blotting immunoprecipitated PSME1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western blot

Detection of Human PSME1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human PSME1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western blot

Detection of Human PSME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for IP at 6 ug/mg lysate. PSME1 was also immunoprecipitated by rabbit anti-PSME1 antibody BL13937. For blotting immunoprecipitated PSME1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human PSME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for IP at 6 ug/mg lysate. PSME1 was also immunoprecipitated by rabbit anti-PSME1 antibody BL13937. For blotting immunoprecipitated PSME1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western blot

Detection of Human PSME1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human PSME1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western blot

Detection of Human PSME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for IP at 6 ug/mg lysate. PSME1 was also immunoprecipitated by rabbit anti-PSME1 antibody BL13937. For blotting immunoprecipitated PSME1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human PSME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for IP at 6 ug/mg lysate. PSME1 was also immunoprecipitated by rabbit anti-PSME1 antibody BL13937. For blotting immunoprecipitated PSME1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western blot

Detection of Human PSME1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human PSME1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Requested From: 
Date Requested: 7/17/2018

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