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Catalog Number Size Price
LS-C757725-200 200 µl $393 
Proteoglycan 4 / Lubricin Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (human cervical epithelial adenocarcinoma cell line) cells labeling PRG4 with PRG4 Antibody (N-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
Proteoglycan 4 / Lubricin Antibody - Anti-PRG4 Antibody (N-Term) at 1:500 dilution + Human liver whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 151 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Proteoglycan 4 / Lubricin Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (human cervical epithelial adenocarcinoma cell line) cells labeling PRG4 with PRG4 Antibody (N-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
Proteoglycan 4 / Lubricin Antibody - Anti-PRG4 Antibody (N-Term) at 1:500 dilution + Human liver whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 151 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Polyclonal Rabbit anti‑Human Proteoglycan 4 / Lubricin Antibody (N‑Terminus, IF, WB) LS‑C757725

Polyclonal Rabbit anti‑Human Proteoglycan 4 / Lubricin Antibody (N‑Terminus, IF, WB) LS‑C757725

Antibody:
Proteoglycan 4 / Lubricin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C757725-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Proteoglycan 4 / Lubricin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Lubricin antibody LS-C757725 is an unconjugated rabbit polyclonal antibody to human Lubricin (Proteoglycan 4) (N-Terminus). Validated for ELISA, IF and WB.
Target
Human Proteoglycan 4 / Lubricin
Synonyms
PRG4 | BG174L6.2 | CACP | HAPO | JCAP | Proteoglycan 4 | Superficial zone proteoglycan | SZP | Lubricin | MSF
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Immunogen
This PRG4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 176-210 amino acids from the human region of human PRG4.
Epitope
N-Terminus
Applications
  • Immunofluorescence (1:25)
  • Western blot (1:500)
  • ELISA
Usage
Western Blot Predicted Molecular Weight: 151.1 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Proteoglycan 4 / Lubricin
Q92954 NM_005807 NP_005798.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024