Human PRKCG / PKC-Gamma
(tested or 100% immunogen sequence identity)
Synthetic phosphopeptide derived from a region of human PKC that contains threonine 514.
Reactivity: Human PKC. Mouse and rat (88% homologous) PKC have not been tested, but are expected to react. PKC,, (75%), I, II, and (63%) may cross-react in cells expressing high levels of these proteins.
Suitable for use in Western blotting applications. For Western blotting applications, we recommend using the antibody at 0.5-1.0 ug/ml. At 0.50 ug/ml, the dilution provides 100 ml working solution, which at 10 ml/blot allows 10 blots to be performed. Positive control: Hela cells treated with PMA, a phorbol ester.
PBS (without Mg2+, Ca2+), 1 mg/ml BSA (IgG, protease free), pH 7.2, 0.1% sodium azide.
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.