Target
Human PRKCA / PKC-Alpha
Synonyms
PRKCA | Aging-associated gene 6 | AAG6 | Alpha PKC | PKC-A | Pkcalpha | Protein kinase C alpha type | PKC Alpha | PKC-alpha | PKCA | Protein kinase c alpha | Protein kinase C, alpha
Reactivity
Human, Rat, Rabbit
(tested or 100% immunogen sequence identity)
Immunogen
Phospho-PKCa (Ser-657/Tyr-658) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around serine 657 and tyrosine 658 of human PKCa. This sequence is similar to a dual phosphorylation site found in PKCbI (Ser-661/Tyr-662). These human sequences are highly conserved in rat and mouse PKCa and PKCbI. In addition, this dual site is homologous to threonine 674 and tyrosine 675 in human PKCg, although this reactivity has not been confirmed.
Specificity
Detects an 82kD protein corresponding to the molecular mass of phosphorylated PKCa on SDS-PAGE immunoblots of neonatal rat brain lysate. Similar results were seen in other PKCa-expressing cells, such as human SKN-SH, endothelial, and HeLa cells, as well as rabbit spleen fibroblasts and rat pituitary cells. In immunoprecipitation experiments with various PKC isoforms, this antibody detected PKCa and PKCb, but not other PKC isoforms in rat brain lysate.