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Catalog Number Size Price
LS-C38529-100 100 µl $604 
Antibody

Polyclonal Rabbit anti‑Human PRKCA / PKC‑Alpha Antibody (phospho‑Ser657/Tyr658, WB) LS‑C38529

Polyclonal Rabbit anti‑Human PRKCA / PKC‑Alpha Antibody (phospho‑Ser657/Tyr658, WB) LS‑C38529

Antibody:
PRKCA / PKC-Alpha Rabbit anti-Human Polyclonal (pSer657/Tyr658) Antibody
Application:
WB, ELISA
Reactivity:
Human, Rat, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$604
LS-C38529-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
PRKCA / PKC-Alpha Rabbit anti-Human Polyclonal (pSer657/Tyr658) Antibody
Application:
WB, ELISA
Reactivity:
Human, Rat, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
PKC-Alpha antibody LS-C38529 is an unconjugated rabbit polyclonal antibody to PKC-Alpha (PRKCA) (pSer657/Tyr658) from human. It is reactive with human, rat and rabbit. Validated for ELISA and WB.
Target
Human PRKCA / PKC-Alpha
Synonyms
PRKCA | Aging-associated gene 6 | AAG6 | Alpha PKC | PKC-A | Pkcalpha | Protein kinase C alpha type | PKC Alpha | PKC-alpha | PKCA | Protein kinase c alpha | Protein kinase C, alpha
Host
Rabbit
Reactivity
Human, Rat, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Phospho-PKCa (Ser-657/Tyr-658) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around serine 657 and tyrosine 658 of human PKCa. This sequence is similar to a dual phosphorylation site found in PKCbI (Ser-661/Tyr-662). These human sequences are highly conserved in rat and mouse PKCa and PKCbI. In addition, this dual site is homologous to threonine 674 and tyrosine 675 in human PKCg, although this reactivity has not been confirmed.
Epitope
pSer657/Tyr658
Specificity
Detects an 82kD protein corresponding to the molecular mass of phosphorylated PKCa on SDS-PAGE immunoblots of neonatal rat brain lysate. Similar results were seen in other PKCa-expressing cells, such as human SKN-SH, endothelial, and HeLa cells, as well as rabbit spleen fibroblasts and rat pituitary cells. In immunoprecipitation experiments with various PKC isoforms, this antibody detected PKCa and PKCb, but not other PKC isoforms in rat brain lysate.
Applications
  • Western blot
  • ELISA
Usage
Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols.
Presentation
PBS, 1 mg/ml BSA, 0.05% Sodium Azide, 50% Glycerol
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKCA / PKC-Alpha
P17252 NM_002737 NP_002728.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/16/2021