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Catalog Number Size Price
LS-B861-50 50 µg (0.1 mg/ml) $460 
PRKCA / PKC-Alpha Antibody - Anti-PRKCA / PKC-Alpha antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC‑plus™ Polyclonal Rabbit anti‑Human PRKCA / PKC‑Alpha Antibody (phospho‑Thr497, IHC) LS‑B861

IHC‑plus™ Polyclonal Rabbit anti‑Human PRKCA / PKC‑Alpha Antibody (phospho‑Thr497, IHC) LS‑B861

Note: This antibody replaces LS-C3383
Antibody:
PRKCA / PKC-Alpha Rabbit anti-Human Polyclonal (pThr497) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B861-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
PRKCA / PKC-Alpha Rabbit anti-Human Polyclonal (pThr497) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PKC-Alpha antibody LS-B861 is an unconjugated rabbit polyclonal antibody to human PKC-Alpha (PRKCA) (pThr497). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human PRKCA / PKC-Alpha
Synonyms
PRKCA | Aging-associated gene 6 | AAG6 | Alpha PKC | PKC-A | Pkcalpha | Protein kinase C alpha type | PKC Alpha | PKC-alpha | PKCA | Protein kinase c alpha | Protein kinase C, alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human PRKCA / PKC-Alpha.
Epitope
pThr497
Specificity
Amino acids 491 to 501 with pTHR497 of human PRKCA
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ELISA (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B861 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B861 was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKCA / PKC-Alpha
P17252 NM_002737 NP_002728.1

Validation

PRKCA / PKC-Alpha Antibody - Anti-PRKCA / PKC-Alpha antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-PRKCA / PKC-Alpha antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-PRKCA / PKC-Alpha antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ PRKCA / PKC-Alpha Antibody (phospho-Thr497) for IHC, ELISA LS-B861 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/22/2021