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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PRKCA / PKC-Alpha Antibody (aa216-237) LS-C17431

PKC-Alpha antibody LS-C17431 is an unconjugated rabbit polyclonal antibody to human PKC-Alpha (PRKCA). Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C17431-100
100 µl
$435
Description
PKC-Alpha antibody LS-C17431 is an unconjugated rabbit polyclonal antibody to human PKC-Alpha (PRKCA). Validated for ELISA, IHC and WB.
Target
Human PRKCA / PKC-Alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1:250)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide corresponding to a distinct C terminal region, (FEKAKLGPAGNKVISPEDRKQ residues 305-325) from human protein kinase C alpha. Percent identity by BLAST analysis: Elephant (95%); Marmoset, Platypus (90%); Turkey, Chicken (86%); Opossum, Xenopus (81%).
Epitope
aa216-237
Specificity
Based on known homologies, it is expected to react with the PKC ortholog in most mammalian species.
Usage
Suitable for use in Western blots, ELISA, and/or Immunohistochemical techniques. For Immunohistochemistry and Western blotting using alkaline phosphatase or HRP, a dilution of 1:250 is recommended. With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further. Optimum dilutions to be determined by researcher.
Presentation
0.05% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PRKCA / PKC-Alpha
P17252 NM_002737 NP_002728.1

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 This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Requested From: United States
Date Requested: 4/21/2019
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