PKC-Alpha antibody LS-C17431 is an unconjugated rabbit polyclonal antibody to human PKC-Alpha (PRKCA). Validated for ELISA, IHC and WB.
Human PRKCA / PKC-Alpha
(tested or 100% immunogen sequence identity)
- Western blot (1:250)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Synthetic peptide corresponding to a distinct C terminal region, (FEKAKLGPAGNKVISPEDRKQ residues 305-325) from human protein kinase C alpha. Percent identity by BLAST analysis: Elephant (95%); Marmoset, Platypus (90%); Turkey, Chicken (86%); Opossum, Xenopus (81%).
Based on known homologies, it is expected to react with the PKC ortholog in most mammalian species.
Suitable for use in Western blots, ELISA, and/or Immunohistochemical techniques. For Immunohistochemistry and Western blotting using alkaline phosphatase or HRP, a dilution of 1:250 is recommended. With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further. Optimum dilutions to be determined by researcher.
0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.