Calcineurin B antibody LS-C155363 is an unconjugated rabbit polyclonal antibody to Calcineurin B (PPP3R1) from human, mouse, rat and other species. Validated for IHC and WB.
Human PPP3R1 / Calcineurin B
Human, Mouse, Rat, Bovine, Chicken
(tested or 100% immunogen sequence identity)
Xenopus (at least 90% immunogen sequence identity)
- IHC - Paraffin (1:100 - 1:1000)
- Western blot (1:500 - 1:3000)
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Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 154 (P63098) of Calcineurin B. Percent identity by BLAST analysis: Human, Mouse, Chicken, Rat, Bovine (100%); Xenopus (97%).
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
For research use only.
About PPP3R1 / Calcineurin B