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Catalog Number Size Price
LS-C747784-20 20 µl $275 
LS-C747784-100 100 µl $389 
POLR1E Antibody - Western blot analysis of extracts of various cell lines, using POLR1E antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
POLR1E Antibody - Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug POLR1E antibody. Western blot was performed from the immunoprecipitate using POLR1E antibody at a dilition of 1:1000.
POLR1E Antibody - Western blot analysis of extracts of various cell lines, using POLR1E antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
POLR1E Antibody - Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug POLR1E antibody. Western blot was performed from the immunoprecipitate using POLR1E antibody at a dilition of 1:1000.
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Polyclonal Rabbit anti‑Human POLR1E Antibody (WB) LS‑C747784

Polyclonal Rabbit anti‑Human POLR1E Antibody (WB) LS‑C747784

Antibody:
POLR1E Rabbit anti-Human Polyclonal Antibody
Application:
WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C747784-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
POLR1E Rabbit anti-Human Polyclonal Antibody
Application:
WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
POLR1E antibody LS-C747784 is an unconjugated rabbit polyclonal antibody to human POLR1E. Validated for IP and WB.
Target
Human POLR1E
Synonyms
POLR1E | PRAF1 | RNA polymerase I subunit A49 | PAF53 | RP11-405L18.3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_071935.1). MAAEVLPSARWQYCGAPDGSQRAVLVQFSNGKLQSPGNMRFTLYENKDSTNPRKRNQRILAAETDRLSYVGNNFGTGALKCNTLCRHFVGILNKTSGQMEVYDAELFNMQPLFSDVSVESELALESQTKTYREKMDSCIEAFGTTKQKRALNTRRMNRVGNESLNRAVAKAAETIIDTKGVTALVSDAIHNDLQDDSLYLPPCYDDAAKPEDVYKFEDLLSPAEYEALQSPSEAFRNVTSEEILKMIEENSHCTFVIEALKSLPSDVESRDRQARCIWFLDTLIKFRAHRVVKRKSALGPGVPHIINTKLLKHFTCLTYNNGRLRNLISDSMKAKITAYVIILALHIHDFQIDLTVLQRDLKLSEKRMMEIAKAMRLKISKRRVS
Applications
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:100)
Usage
The predicted MW is 47kDa/53kDa, while the observed MW by Western blot was 56kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLR1E
Q9GZS1 NM_022490 NP_071935.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024