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POLR1E

polymerase (RNA) I polypeptide E, 53kDa

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.

Gene Name: polymerase (RNA) I polypeptide E, 53kDa
Synonyms: POLR1E, PRAF1, RNA polymerase I subunit A49, PAF53, RP11-405L18.3
Target Sequences: NM_022490 NP_071935.1 Q9GZS1

☰ Filters
Antibodies (20)
Proteins (4)
Over-Expression Lysate (2)
Primary (20)
Recombinant (2)
POLR1E (24)
Human (20)
Mouse (4)
Rat (3)
Bovine (3)
Chimpanzee (3)
Dog (3)
Gibbon (3)
Guinea pig (3)
Hamster (3)
Horse (3)
Monkey (3)
Pig (3)
IHC (4)
IHC-P (1)
WB (17)
ELISA (11)
IF (1)
IP (4)
Peptide-ELISA (1)
rabbit (20)
IgG (14)
polyclonal pc (20)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (12)
aa171-198 (7)
aa35-84 (4)
Internal (1)
aa225-275 (1)
aa431-481 (1)
No (24)
GST (1)
Myc-DDK (Flag) (3)
Human (3)
293T Cells (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (1)
POLR1E Antibody - Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using POLR1E antibody.
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POLR1E Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
100 µg/$357
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POLR1E Rabbit anti-Human Polyclonal (Internal) Antibody
Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
POLR1E Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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POLR1E Rabbit anti-Human Polyclonal Antibody
Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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POLR1E Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IP, WB
Unconjugated
100 µg/$331
POLR1E Antibody - Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue  using POLR1E Polyclonal Antibody at dilution of 1:40(×200)
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POLR1E Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
POLR1E Antibody - Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue  using POLR1E Polyclonal Antibody at dilution of 1:40(×200)
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POLR1E Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
Select
POLR1E Rabbit anti-Human Polyclonal (aa171-198) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
Select
POLR1E Rabbit anti-Human Polyclonal (aa171-198) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
Select
POLR1E Rabbit anti-Human Polyclonal (aa171-198) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
Select
POLR1E Rabbit anti-Human Polyclonal (aa171-198) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
Select
POLR1E Rabbit anti-Human Polyclonal (aa171-198) (Azide-free) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
Select
POLR1E Rabbit anti-Human Polyclonal (aa171-198) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
POLR1E Antibody - Detection of human POLR1E/PRAF1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-POLR1E/PRAF1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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POLR1E Rabbit anti-Human Polyclonal (aa225-275) Antibody
Human
IP, WB
Unconjugated
100 µl/$578
POLR1E Antibody - Western blot analysis of extracts of various cell lines, using POLR1E antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
Select
POLR1E Rabbit anti-Human Polyclonal Antibody
Human
IP, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
POLR1E Antibody - POLR1E / PRAF1 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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POLR1E Rabbit anti-Human Polyclonal (aa35-84) Antibody
Mouse, Human
WB
Unconjugated
100 µl/$424
POLR1E Antibody - POLR1E / PRAF1 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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POLR1E Rabbit anti-Human Polyclonal (aa35-84) (HRP) Antibody
Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Gibbon, Chimpanzee, Human, Monkey
WB
HRP Conjugated
100 µl/$469
POLR1E Antibody - POLR1E / PRAF1 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
POLR1E Rabbit anti-Human Polyclonal (aa35-84) (Biotin) Antibody
Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Gibbon, Chimpanzee, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
POLR1E Antibody - POLR1E / PRAF1 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
POLR1E Rabbit anti-Human Polyclonal (aa35-84) (FITC) Antibody
Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Gibbon, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100 µl/$469
POLR1E Antibody - POLR1E Antibody western blot of K562 cell line lysates (35 ug/lane). The POLR1E antibody detected the POLR1E protein (arrow).
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POLR1E Rabbit anti-Human Polyclonal (aa171-198) Antibody
Human
WB
Unconjugated
400 µl/$393
Select
POLR1E Rabbit anti-Human Polyclonal (aa431-481) Antibody
Human
IP
Unconjugated
10 µg/$295; 100 µl/$514
POLR1E Protein - Purified recombinant protein POLR1E was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
47.1 kDa
20 µg/$1,107
POLR1E Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
47.1 kDa
20 µg/$150
POLR1E Protein - 12.5% SDS-PAGE of human PRAF1 stained with Coomassie Blue
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Wheat Germ Extract
GST
73.7 kDa
2 µg/$439
POLR1E Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
47.1 kDa
100 µg/$494
Viewing 1-24 of 24 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).