Human PODXL / Podocalyxin

Mouse

Human, Rat, Rabbit (tested or 100% immunogen sequence identity)

IgM Monoclonal

2A4

Unconjugated

PEG purified

Ready-to-Use. Also available Unmodified or Azide-free, BSA-free.

Recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human PODXL.

Extracellular Domain

Human PODXL / Podocalyxin

• IHC
• IHC - Paraffin (1:1)
• Immunofluorescence
• Flow Cytometry

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. Optimal dilution of the Podocalyxin antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O00592 NCBI: NM_005397 NP_005388.2