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Catalog Number Size Price
LS-C804405-100 100 µl (1 mg/ml) $379 
LS-C804405-200 200 µl (1 mg/ml) $421 
PMEL / SILV / gp100 Antibody - 1:100 staining human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PMEL / SILV / gp100 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PMEL / SILV / gp100 Antibody - Western blot analysis of PMEL17/GP100 using 293 whole cells lysates
PMEL / SILV / gp100 Antibody - 1:100 staining human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PMEL / SILV / gp100 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PMEL / SILV / gp100 Antibody - Western blot analysis of PMEL17/GP100 using 293 whole cells lysates
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Polyclonal Rabbit anti‑Human PMEL / SILV / gp100 Antibody (IHC, IF, WB) LS‑C804405

Polyclonal Rabbit anti‑Human PMEL / SILV / gp100 Antibody (IHC, IF, WB) LS‑C804405

Antibody:
PMEL / SILV / gp100 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C804405-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PMEL / SILV / gp100 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Gp100 antibody LS-C804405 is an unconjugated rabbit polyclonal antibody to gp100 (PMEL / SILV) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human PMEL / SILV / gp100
Synonyms
PMEL | gp100 | Melanocyte protein PMEL | Me20m/me20s | p100 | PMEL17 | Premelanosome protein | SIL | Silver homolog (mouse) | Silver, mouse, homolog of | Silver locus protein homolog | ME20-M | Me20-m/me20-s | ME20M | Melanocyte protein mel 17 | Melanocyte protein Pmel 17 | Silver (mouse homolog) like | D12S53E | ME20 | Melanosomal matrix protein17 | SILV
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human PMEL17 / GP100.
Specificity
PMEL17 / GP100 Antibody detects endogenous levels of total PMEL17 / GP100.
Applications
  • IHC
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:1000 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PMEL / SILV / gp100
P40967 NM_006928 NP_008859.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/30/2024