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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PKG / PRKG1 Antibody (clone 2B3) IHC‑plus™ LS‑B4398
Note: This antibody replaces LS-C105014
PRKG1 antibody LS-B4398 is an unconjugated mouse monoclonal antibody to human PRKG1 (PKG). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4398-50
50 µg (0.5 mg/ml)
$485

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Product Description

PRKG1 antibody LS-B4398 is an unconjugated mouse monoclonal antibody to human PRKG1 (PKG). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About PKG / PRKG1
Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Q13976 NM_006258 NP_006249.1

PRKG1 Antibody, CGKI Antibody, CGMP kinase I Antibody, CGMP protein kinase type I Antibody, CGK-I Antibody, CGK1 Antibody, PKGI Antibody, PKG I Antibody, PRKGR1B Antibody, PRKG1B Antibody, PRKGR1A Antibody, PGK Antibody, PKG Antibody, CGK Antibody, CGK 1 Antibody, CGKI-alpha Antibody, CGKI-BETA Antibody


Specifications

Target
Human PKG / PRKG1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [2B3]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PKG / PRKG1 antibody was raised against pRKG1 (NP_006249, 73 a.a. ~ 171 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Usage
Western blot performed using the immunogenic protein. Sandwich ELISA (Recombinant protein).
Presentation
PBS, pH 7.4
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-PRKG1 / CGKI antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4398 concentration 5 ug/ml.
Anti-PRKG1 / CGKI antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4398 concentration 5 ug/ml.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PRKG1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PRKG1 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-PRKG1 / CGKI antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4398 concentration 5 ug/ml.
Anti-PRKG1 / CGKI antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4398 concentration 5 ug/ml.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PRKG1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PRKG1 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-PRKG1 / CGKI antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4398 concentration 5 ug/ml.
Anti-PRKG1 / CGKI antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4398 concentration 5 ug/ml.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PRKG1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PRKG1 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Anti-PRKG1 / CGKI antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4398 concentration 5 ug/ml.
Anti-PRKG1 / CGKI antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4398 concentration 5 ug/ml.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged PRKG1 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PRKG1 is approximately 0.1 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 8/19/2018

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