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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PKG / PRKG1 Antibody (aa1‑300) LS‑C186222
PRKG1 antibody LS-C186222 is an unconjugated rabbit polyclonal antibody to human PRKG1 (PKG). Validated for WB.
Catalog
Size
Price
LS-C186222-100
100 µl (1 mg/ml)
$335

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Product Description

PRKG1 antibody LS-C186222 is an unconjugated rabbit polyclonal antibody to human PRKG1 (PKG). Validated for WB.
About PKG / PRKG1
Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Q13976 NM_006258 NP_006249.1

PRKG1 Antibody, CGKI Antibody, CGMP kinase I Antibody, CGMP protein kinase type I Antibody, CGK-I Antibody, CGK1 Antibody, PKGI Antibody, PKG I Antibody, PRKGR1B Antibody, PRKG1B Antibody, PRKGR1A Antibody, PGK Antibody, PKG Antibody, CGK Antibody, CGK 1 Antibody, CGKI-alpha Antibody, CGKI-BETA Antibody


Specifications

Target
Human PKG / PRKG1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine, Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:5000 - 1:20000)
Immunogen
PKG / PRKG1 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 300 of cGK1 alpha (SwissProt Q13976). Percent identity by BLAST analysis: Human (100%); Pig, Bovine (99%); Rabbit, Rat (98%).
Epitope
aa1-300
Specificity
Human PKG / PRKG1
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

Sample (30 ug of whole cell lysate). A: JC, B: BCL-1. 7.5% SDS PAGE. PKG / PRKG1 antibody diluted at 1:10000.
Sample (30 ug of whole cell lysate). A: JC, B: BCL-1. 7.5% SDS PAGE. PKG / PRKG1 antibody diluted at 1:10000.

Western blot

Sample (30 ug of whole cell lysate). A: JC, B: BCL-1. 7.5% SDS PAGE. PKG / PRKG1 antibody diluted at 1:10000.
Sample (30 ug of whole cell lysate). A: JC, B: BCL-1. 7.5% SDS PAGE. PKG / PRKG1 antibody diluted at 1:10000.

Western blot

Sample (30 ug of whole cell lysate). A: JC, B: BCL-1. 7.5% SDS PAGE. PKG / PRKG1 antibody diluted at 1:10000.
Sample (30 ug of whole cell lysate). A: JC, B: BCL-1. 7.5% SDS PAGE. PKG / PRKG1 antibody diluted at 1:10000.

Western blot

Sample (30 ug of whole cell lysate). A: JC, B: BCL-1. 7.5% SDS PAGE. PKG / PRKG1 antibody diluted at 1:10000.
Sample (30 ug of whole cell lysate). A: JC, B: BCL-1. 7.5% SDS PAGE. PKG / PRKG1 antibody diluted at 1:10000.

Requested From: United States
Date Requested: 8/15/2018

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