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Catalog Number Size Price
LS-C497170-20 20 µl $275 
LS-C497170-100 100 µl $389 
PHGDH Antibody - Immunohistochemistry of paraffin-embedded rat brain using PHGDH antibodyat dilution of 1:100 (40x lens).
PHGDH Antibody - Immunohistochemistry of paraffin-embedded rat lung using PHGDH antibodyat dilution of 1:100 (40x lens).
PHGDH Antibody - Western blot analysis of extracts of various cell lines, using PHGDH antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
PHGDH Antibody - Immunohistochemistry of paraffin-embedded rat brain using PHGDH antibodyat dilution of 1:100 (40x lens).
PHGDH Antibody - Immunohistochemistry of paraffin-embedded rat lung using PHGDH antibodyat dilution of 1:100 (40x lens).
PHGDH Antibody - Western blot analysis of extracts of various cell lines, using PHGDH antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
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Polyclonal Rabbit anti‑Human PHGDH Antibody (IHC, WB) LS‑C497170

Polyclonal Rabbit anti‑Human PHGDH Antibody (IHC, WB) LS‑C497170

Antibody:
PHGDH Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C497170-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PHGDH Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PHGDH antibody LS-C497170 is an unconjugated rabbit polyclonal antibody to PHGDH from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human PHGDH
Synonyms
PHGDH | 3-PGDH | 3PGDH | PGDH | Phosphoglycerate dehydrogenase | PDG | PGAD | PGDH3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 264-533 of human PHGDH (NP_006614.2). EEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNGAVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHVTEAFQFHF
Applications
  • IHC
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 56kDa, while the observed MW by Western blot was 57kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PHGDH
O43175 NM_006623 NP_006614.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024