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Catalog Number Size Price
LS-C791008-100 100 µg $511 
PHGDH Antibody - Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PHGDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
PHGDH Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY PHGDH. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHGDH.
PHGDH Antibody - Equivalent amounts of cell lysates  and PHGDH-Knockout Hela cells  were separated by SDS-PAGE and immunoblotted with anti-PHGDH monoclonal antibody(1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody  as a loading control.
PHGDH Antibody - Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PHGDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
PHGDH Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY PHGDH. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHGDH.
PHGDH Antibody - Equivalent amounts of cell lysates  and PHGDH-Knockout Hela cells  were separated by SDS-PAGE and immunoblotted with anti-PHGDH monoclonal antibody(1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody  as a loading control.
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Monoclonal Mouse anti‑Human PHGDH Antibody (clone OTI5C4, Carrier‑free, IHC, WB) LS‑C791008

Monoclonal Mouse anti‑Human PHGDH Antibody (clone OTI5C4, Carrier‑free, IHC, WB) LS‑C791008

Antibody:
PHGDH Mouse anti-Human Monoclonal (Carrier-free) (OTI5C4) Antibody
Application:
IHC, WB
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$511
LS-C791008-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PHGDH Mouse anti-Human Monoclonal (Carrier-free) (OTI5C4) Antibody
Application:
IHC, WB
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
PHGDH antibody LS-C791008 is an unconjugated mouse monoclonal antibody to human PHGDH. Validated for IHC and WB.
Target
Human PHGDH
Synonyms
PHGDH | 3-PGDH | 3PGDH | PGDH | Phosphoglycerate dehydrogenase | PDG | PGAD | PGDH3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
OTI5C4
Conjugations
Unconjugated
Purification
Purified from ascites.
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Full length human recombinant protein of human PHGDH (NP_006614) produced in HEK293T cell.
Applications
  • IHC (1:150)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PHGDH
O43175 NM_006623 NP_006614.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024