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Catalog Number Size Price
LS-C392982-200 200 µl $393 
PHC3 Antibody - Antibody staining PHC3 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
PHC3 Antibody - All lanes: Anti-PHC3 Antibody (C-Term) at 1:2000 dilution. Lane 1: HeLa whole cell lysate. Lane 2: SH-SY5Y whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: U-2OS whole cell lysate. Lane 5: A549 whole cell lysate. Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 106 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
PHC3 Antibody - Antibody staining PHC3 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
PHC3 Antibody - All lanes: Anti-PHC3 Antibody (C-Term) at 1:2000 dilution. Lane 1: HeLa whole cell lysate. Lane 2: SH-SY5Y whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: U-2OS whole cell lysate. Lane 5: A549 whole cell lysate. Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 106 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Polyclonal Rabbit anti‑Human PHC3 Antibody (aa819‑850, IHC, WB) LS‑C392982

Polyclonal Rabbit anti‑Human PHC3 Antibody (aa819‑850, IHC, WB) LS‑C392982

Antibody:
PHC3 Rabbit anti-Human Polyclonal (aa819-850) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C392982-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PHC3 Rabbit anti-Human Polyclonal (aa819-850) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PHC3 antibody LS-C392982 is an unconjugated rabbit polyclonal antibody to human PHC3 (aa819-850). Validated for IHC, Peptide-ELISA and WB.
Target
Human PHC3
Synonyms
PHC3 | EDR3 | Early development regulator 3 | Polyhomeotic like 3 | PH3 | Polyhomeotic-like protein 3 | Homolog of polyhomeotic 3 | HPH3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa819-850
Specificity
This PHC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 819-850 amino acids from human PHC3.
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • Western blot (1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PHC3
Q8NDX5 NM_024947 NP_079223.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024