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Catalog Number Size Price
LS-C744612-25 25 µl (90 mg/ml) $304 
PECAM-1 / CD31 Antibody - Immunohistochemistry with anti-CD31 antibody showing CD31 staining of vascular endothelium in human colon at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-human CD31 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3’3-diamino-benzidine substrate (DAB).
PECAM-1 / CD31 Antibody - Western Blot of rabbit Anti-CD31 Antibody. Lane 1: 3T3 Whole cell lysate. Lane 2: Jurkat Whole cell lysate. Lane 3: HT-29 Whole cell lysate. Load: 10µg/lane. Primary Antibody Anti-CD-31 used 1:500 with 0.75% TBS with Casein overnight. Secondary Antibody Goat anti-rabbit HRP used 1:40,000 for 30 min at room temp. Expect MW: 80kda.
PECAM-1 / CD31 Antibody - Immunohistochemistry with anti-CD31 antibody showing CD31 staining of vascular endothelium in human colon at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-human CD31 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3’3-diamino-benzidine substrate (DAB).
PECAM-1 / CD31 Antibody - Western Blot of rabbit Anti-CD31 Antibody. Lane 1: 3T3 Whole cell lysate. Lane 2: Jurkat Whole cell lysate. Lane 3: HT-29 Whole cell lysate. Load: 10µg/lane. Primary Antibody Anti-CD-31 used 1:500 with 0.75% TBS with Casein overnight. Secondary Antibody Goat anti-rabbit HRP used 1:40,000 for 30 min at room temp. Expect MW: 80kda.
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Polyclonal Rabbit anti‑Rat PECAM‑1 / CD31 Antibody (C‑Terminus, IHC, WB) LS‑C744612

Polyclonal Rabbit anti‑Rat PECAM‑1 / CD31 Antibody (C‑Terminus, IHC, WB) LS‑C744612

Antibody:
PECAM-1 / CD31 Rabbit anti-Rat Polyclonal (C-Terminus) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Rat, Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C744612-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PECAM-1 / CD31 Rabbit anti-Rat Polyclonal (C-Terminus) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Rat, Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CD31 antibody LS-C744612 is an unconjugated rabbit polyclonal antibody to CD31 (PECAM-1) (C-Terminus) from rat. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Target
Rat PECAM-1 / CD31
Synonyms
PECAM1 | CD31 | CD31 antigen | EndoCAM | PECA1 | PECAM-1 | GPIIA
Host
Rabbit
Reactivity
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Human, Mouse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of rat CD31 protein.
Epitope
C-Terminus
Specificity
A BLAST analysis was used to suggest cross-reactivity with CD31 from rat, human, and mouse based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
  • IHC (4 µg/ml)
  • Western blot (1:500)
  • ELISA (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PECAM-1 / CD31
P16284 NM_000442 NP_000433.4

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/29/2024