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Catalog Number Size Price
LS-C96364-300 300 µg $830 
Antibody

Monoclonal Rat anti‑Mouse PECAM‑1 / CD31 Antibody (clone ER‑MP12, Biotin, IHC) LS‑C96364

Monoclonal Rat anti‑Mouse PECAM‑1 / CD31 Antibody (clone ER‑MP12, Biotin, IHC) LS‑C96364

Antibody:
PECAM-1 / CD31 Rat anti-Mouse Monoclonal (Biotin) (ER-MP12) Antibody
Application:
IHC, IHC-P, IHC-Fr
Reactivity:
Mouse
Format:
Biotin, Unmodified
Price
Catalog Number
$830
LS-C96364-300
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PECAM-1 / CD31 Rat anti-Mouse Monoclonal (Biotin) (ER-MP12) Antibody
Application:
IHC, IHC-P, IHC-Fr
Reactivity:
Mouse
Format:
Biotin, Unmodified

Specifications

Description
CD31 antibody LS-C96364 is a biotin-conjugated rat monoclonal antibody to mouse CD31 (PECAM-1). Validated for IHC.
Target
Mouse PECAM-1 / CD31
Synonyms
PECAM1 | CD31 | CD31 antigen | EndoCAM | PECA1 | PECAM-1 | GPIIA
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Clone
ER-MP12
Conjugations
Biotin
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Murine macrophage precursor cells
Specificity
The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 140kD surface glycoprotein (reducing and non reducing conditions) identified as CD31. The antigen expression closely follows that of CD34. Antigen distribution: Isolated cells: The antigen is found on the surface of approximately 40% of freshly isolated bone marrow cells of adult mice, on the majority of immortalized macrophage precursor cell lines (M1, RMB-1, RMB-3) corresponding to CFU-GM cells. The antibody detects pluri- and multipotent stem cells and prothymocytes as well as particular lymphoid cells in bone marrow and peripheral lymphoid organs. Tissue sections: The antigen is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express the antigen. Specificity: Mouse: Major population of colony forming unit macrophage (CFU-M) precursor cells, subpopulation of pre CFU-M and monoblasts, precursor cells of granulocytes. Other: unknown
Applications
  • IHC
  • IHC - Paraffin (1:40)
  • IHC - Frozen (1:1600)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant. For IHC with paraffin embedded sections antigen retrieval with Proteinase K is recommended. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, 5 mg/ml BSA, 0.05% Kathon
Reconstitution
Reconstitute with 0.5ml distilled water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PECAM-1 / CD31
P16284 NM_000442 NP_000433.4

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/19/2024