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Catalog Number Size Price
LS-C331251-20 20 µl $275 
LS-C331251-100 100 µl $389 
PDIA3 / ERp57 Antibody - Immunohistochemistry of paraffin-embedded mouse brain using PDIA3 antibodyat dilution of 1:100 (40x lens).
PDIA3 / ERp57 Antibody - Immunohistochemistry of paraffin-embedded human stomach using PDIA3 antibodyat dilution of 1:100 (40x lens).
PDIA3 / ERp57 Antibody - Western blot analysis of extracts of various cell lines, using PDIA3 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
PDIA3 / ERp57 Antibody - Immunohistochemistry of paraffin-embedded mouse brain using PDIA3 antibodyat dilution of 1:100 (40x lens).
PDIA3 / ERp57 Antibody - Immunohistochemistry of paraffin-embedded human stomach using PDIA3 antibodyat dilution of 1:100 (40x lens).
PDIA3 / ERp57 Antibody - Western blot analysis of extracts of various cell lines, using PDIA3 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
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Polyclonal Rabbit anti‑Human PDIA3 / ERp57 Antibody (IHC, WB) LS‑C331251

Polyclonal Rabbit anti‑Human PDIA3 / ERp57 Antibody (IHC, WB) LS‑C331251

Antibody:
PDIA3 / ERp57 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C331251-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PDIA3 / ERp57 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ERp57 antibody LS-C331251 is an unconjugated rabbit polyclonal antibody to ERp57 (PDIA3) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human PDIA3 / ERp57
Synonyms
PDIA3 | 58 kDa microsomal protein | ER protein 57 | ERp60 | ERp61 | Disulfide isomerase ER-60 | Endoplasmic reticulum P58 | GRP58 | HsT17083 | p58 | Phospholipase C-alpha | PI-PLC | ER protein 60 | ER60 | ERp57 | GRP57 | Protein disulfide-isomerase A3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 206-505 of human PDIA3 (NP_005304.3). FRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL
Specificity
Human PDIA3 / ERp57
Applications
  • IHC (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 56kDa, while the observed MW by Western blot was 57kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDIA3 / ERp57
P30101 NM_005313 NP_005304.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/5/2024