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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDIA3 / ERp57

protein disulfide isomerase family A, member 3

PDIA3 / ERp57 encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Gene Name: protein disulfide isomerase family A, member 3
Synonyms: PDIA3, 58 kDa microsomal protein, ER protein 57, ERp60, ERp61, Disulfide isomerase ER-60, Endoplasmic reticulum P58, GRP58, HsT17083, p58, Phospholipase C-alpha, PI-PLC, ER protein 60, ER60, ERp57, GRP57, Protein disulfide-isomerase A3
Target Sequences: NM_005313 NP_005304.3 P30101

Filtered by: None
Target
PDIA3 / ERp57 (71)
Reactivity
Human (71)
Mouse (37)
Rat (39)
Bat (7)
Bovine (22)
Chimpanzee (6)
Dog (33)
Gibbon (6)
Goat (2)
Guinea pig (30)
Hamster (32)
Horse (10)
Monkey (35)
Orangutan (6)
Pig (33)
Rabbit (25)
Sheep (2)
Zebrafish (4)
Application
IHC (42)
IHC-Fr (2)
IHC-P (30)
WB (71)
Flo (5)
ELISA (9)
ICC (2)
IF (6)
IP (22)
Peptide-ELISA (1)
Host
rabbit (37)
mouse (29)
goat (5)
Isotype
IgG (18)
IgG1 (24)
IgG2a (2)
IgG2a,k (2)
IgG2b (1)
Clonality
monoclonal mc (29)
polyclonal pc (42)
Clone
2D2 (1)
3D2 (1)
3E1 (1)
3H5 (1)
4D7 (1)
AT9E9 (1)
Map.ERP57 (17)
MaP.Erp57 (1)
MaP.ERp57 (1)
PAT9E9AT (1)
Format
AP Conjugated (1)
APC (1)
APC Conjugated (2)
Atto 390 Conjugated (1)
Atto 488 Conjugated (1)
Atto 565 Conjugated (1)
Atto 594 Conjugated (2)
Atto 633 Conjugated (1)
Atto 655 Conjugated (1)
Atto 680 Conjugated (1)
Atto 700 Conjugated (1)
Biotin Conjugated (5)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (5)
HRP Conjugated (4)
PE (1)
PE Conjugated (1)
PerCP Conjugated (1)
RPE Conjugated (1)
SA Conjugated (1)
Unconjugated (39)
Epitope
aa162-416 (8)
C-Terminus (6)
aa82-131 (4)
aa140-505 (2)
111-160 aa, Internal (1)
Internal (1)
aa172-188 (1)
aa192-220 (1)
aa25-75 (1)
aa446-475 (1)
aa471-505 (1)
aa492-505 (1)
aa50-100 (1)
Publications
No (71)
Showing 1-25 of 71 results

PDIA3 / ERp57 Antibody (C-Terminus) IHC-plus™ LS-B9768
Goat Polyclonal (IgG) to Human PDIA3 / ERp57
Mouse, Dog, Rat, Human, Monkey
IF IHC IHC-P WB
Unconjugated
100µl 3mg/ml  $425.00
Anti-PDIA3 / ERp57 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence. Anti-ERP57 antibody in primary RPE cells at 1:100 dilution. Cells were fixed with 4% of PFA.

PDIA3 / ERp57 Antibody (C-Terminus) IHC-plus™ LS-B9769
Goat Polyclonal (IgG) to Human PDIA3 / ERp57
Mouse, Dog, Rat, Human, Monkey
IF IHC IHC-P WB
Unconjugated
200µg 100µl 2mg/ml  $425.00
Anti-PDIA3 / ERp57 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence. Anti-ERP57 antibody in primary RPE cells at 1:100 dilution. Cells were fixed with 4% of PFA.

PDIA3 / ERp57 Antibody IHC-plus™ LS-B3699
Rabbit Polyclonal to Rat PDIA3 / ERp57
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Human, Monkey
IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-PDIA3 / ERp57 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-PDIA3 / ERp57 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

PDIA3 / ERp57 Antibody (aa192-220) LS-C163484
Rabbit Polyclonal to Human PDIA3 / ERp57
Human
Flo IHC IHC-P WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of PDIA3 Antibody in A2058, A375, Ramos cell line lysates (35 ug/lane). PDIA3 (arrow) was detected using the purified antibody.(2 ug/ml)

PDIA3 / ERp57 Antibody (clone Map.ERP57) IHC-plus™ LS-B7455
Mouse Monoclonal [clone Map.ERP57] (IgG1) to Human PDIA3 / ERp57
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Human, Monkey
IHC IHC-P IP WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-PDIA3 / ERp57 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of PDIA3 / ERp57 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PDIA3 / ERp57 Antibody (clone Map.ERP57, Atto 488) LS-C230574
Mouse Monoclonal [clone Map.ERP57] (IgG1) to Human PDIA3 / ERp57
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Human, Monkey
IHC IHC-P IP WB
Atto 488 Conjugated
200µg 1mg/ml  $435.00
Anti-PDIA3 / ERp57 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of PDIA3 / ERp57 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PDIA3 / ERp57 Antibody (clone Map.ERP57, Atto 565) LS-C230575
Mouse Monoclonal [clone Map.ERP57] (IgG1) to Human PDIA3 / ERp57
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Human, Monkey
IHC IHC-P IP WB
Atto 565 Conjugated
200µg 1mg/ml  $435.00
Anti-PDIA3 / ERp57 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of PDIA3 / ERp57 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PDIA3 / ERp57 Antibody (clone Map.ERP57, Atto 594) LS-C230576
Mouse Monoclonal [clone Map.ERP57] (IgG1) to Human PDIA3 / ERp57
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Human, Monkey
IHC IHC-P IP WB
Atto 594 Conjugated
200µg 1mg/ml  $435.00
Anti-PDIA3 / ERp57 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of PDIA3 / ERp57 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PDIA3 / ERp57 Antibody (clone Map.ERP57, Atto 633) LS-C230577
Mouse Monoclonal [clone Map.ERP57] (IgG1) to Human PDIA3 / ERp57
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Human, Monkey
IHC IHC-P IP WB
Atto 633 Conjugated
200µg 1mg/ml  $435.00
Anti-PDIA3 / ERp57 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of PDIA3 / ERp57 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PDIA3 / ERp57 Antibody (clone Map.ERP57, Atto 655) LS-C230578
Mouse Monoclonal [clone Map.ERP57] (IgG1) to Human PDIA3 / ERp57
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Human, Monkey
IHC IHC-P IP WB
Atto 655 Conjugated
200µg 1mg/ml  $435.00
Anti-PDIA3 / ERp57 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of PDIA3 / ERp57 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PDIA3 / ERp57 Antibody (clone Map.ERP57, Atto 680) LS-C230579
Mouse Monoclonal [clone Map.ERP57] (IgG1) to Human PDIA3 / ERp57
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Human, Monkey
IHC IHC-P IP WB
Atto 680 Conjugated
200µg 1mg/ml  $435.00
Anti-PDIA3 / ERp57 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of PDIA3 / ERp57 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PDIA3 / ERp57 Antibody (clone Map.ERP57, APC) LS-C230581
Mouse Monoclonal [clone Map.ERP57] (IgG1) to Human PDIA3 / ERp57
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Human, Monkey
IHC IHC-P IP WB
APC Conjugated
200µg 1mg/ml  $435.00
Anti-PDIA3 / ERp57 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of PDIA3 / ERp57 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PDIA3 / ERp57 Antibody (clone Map.ERP57, AP) LS-C230580
Mouse Monoclonal [clone Map.ERP57] (IgG1) to Human PDIA3 / ERp57
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Human, Monkey
IHC IHC-P IP WB
AP Conjugated
200µg 1mg/ml  $425.00
Anti-PDIA3 / ERp57 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of PDIA3 / ERp57 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PDIA3 / ERp57 Antibody LS-C754805
Rabbit Polyclonal (IgG) to Human PDIA3 / ERp57
Human
ELISA Flo IF IHC IP WB
Unconjugated
100µg  $335.00

PDIA3 / ERp57 Antibody (C-Terminus) LS-C146188
Rabbit Polyclonal to Human PDIA3 / ERp57
Zebrafish, Pig, Horse, Rat, Hamster, Dog, Guinea pig, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
PDIA3 / ERp57 antibody Western blot of Fetal Kidney lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

PDIA3 / ERp57 Antibody (aa446-475) LS-C163483
Rabbit Polyclonal to Human PDIA3 / ERp57
Human
Flo IHC IHC-P WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of PDIA3 Antibody in A2058 cell line lysates (35 ug/lane). PDIA3 (arrow) was detected using the purified antibody.

PDIA3 / ERp57 Antibody (clone PAT9E9AT) LS-C760716
Mouse Monoclonal [clone PAT9E9AT] (IgG2a,k) to Human PDIA3 / ERp57
Human
ELISA Flo ICC WB
Unconjugated
5µg  $195.00; 20µg  $255.00; 100µg  $475.00

PDIA3 / ERp57 Antibody (aa140-505, clone 4D7) LS-C337796
Mouse Monoclonal [clone 4D7] (IgG2a) to Human PDIA3 / ERp57
Human
IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Human Kidney tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
IHC of paraffin-embedded Human liver tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

PDIA3 / ERp57 Antibody (clone 3E1) LS-C174083
Mouse Monoclonal [clone 3E1] (IgG2b) to Human PDIA3 / ERp57
Human
IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Human breast tissue using anti-PDIA3 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-PDIA3 mouse monoclonal antibody.

PDIA3 / ERp57 Antibody (clone 3H5) LS-C174089
Mouse Monoclonal [clone 3H5] (IgG2a) to Human PDIA3 / ERp57
Human
IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Human Kidney tissue using anti-PDIA3 mouse monoclonal antibody.
IHC of paraffin-embedded Human liver tissue using anti-PDIA3 mouse monoclonal antibody.

PDIA3 / ERp57 Antibody LS-C757215
Goat Polyclonal (IgG) to Human PDIA3 / ERp57
Human
IF IHC-Fr IHC-P WB
Unconjugated
1mg  $515.00

PDIA3 / ERp57 Antibody LS-C757217
Goat Polyclonal (IgG) to Human PDIA3 / ERp57
Human
IF IHC-Fr IHC-P WB
Unconjugated
1.5mg  $515.00

PDIA3 / ERp57 Antibody LS-C496635
Rabbit Polyclonal (IgG) to Human PDIA3 / ERp57
Human
Flo IHC WB
Unconjugated
100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of A2058 cells, using PDIA3 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

PDIA3 / ERp57 Antibody (aa172-188) LS-C312831
Rabbit Polyclonal to Human PDIA3 / ERp57
Mouse, Goat, Guinea pig, Sheep, Bovine, Rat, Pig, Horse, Bat, Human, Monkey
IHC IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $355.00
PDIA3 / ERp57 antibody. IHC(P): Human Placenta Tissue.
WB of PDIA3 / ERp57 antibody. Lane 1: SMMC Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: U87 Cell Lysate. Lane 4: HELA Cell Lysate. Lane 5: MCF-7 Cell Lysate.

PDIA3 / ERp57 Antibody (aa471-505) LS-C407984
Rabbit Polyclonal to Human PDIA3 / ERp57
Mouse, Bat, Human
IHC IHC-P WB
Unconjugated
100µg  $355.00
ERp57 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
IHC analysis of ERp57 using anti-ERp57 antibody. ERp57 was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERp57 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Showing 1-25 of 71 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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