Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C774004-100 100 µg (1 mg/ml) $479 
PDIA3 / ERp57 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ERp57 Antibody. (D) Composite.
PDIA3 / ERp57 Antibody - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 57 kDa Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone 4F9. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 57 kDa.
PDIA3 / ERp57 Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ERp57 Antibody. (D) Composite.
PDIA3 / ERp57 Antibody - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 57 kDa Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone 4F9. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 57 kDa.
1 of 2
2 of 2

Monoclonal Mouse anti‑Human PDIA3 / ERp57 Antibody (clone 4F9, Atto 488, IF, WB) LS‑C774004

Monoclonal Mouse anti‑Human PDIA3 / ERp57 Antibody (clone 4F9, Atto 488, IF, WB) LS‑C774004

Antibody:
PDIA3 / ERp57 Mouse anti-Human Monoclonal (Atto 488) (4F9) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Atto 488, Unmodified
Other formats:
Price
Catalog Number
$479
LS-C774004-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PDIA3 / ERp57 Mouse anti-Human Monoclonal (Atto 488) (4F9) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Atto 488, Unmodified
Other formats:

Specifications

Description
ERp57 antibody LS-C774004 is an Atto 488-conjugated mouse monoclonal antibody to human ERp57 (PDIA3). Validated for IF and WB.
Target
Human PDIA3 / ERp57
Synonyms
PDIA3 | 58 kDa microsomal protein | ER protein 57 | ERp60 | ERp61 | Disulfide isomerase ER-60 | Endoplasmic reticulum P58 | GRP58 | HsT17083 | p58 | Phospholipase C-alpha | PI-PLC | ER protein 60 | ER60 | ERp57 | GRP57 | Protein disulfide-isomerase A3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
4F9
Conjugations
Atto 488. Also available conjugated with APC, Atto 390, Atto 594, Biotin, FITC, PerCP, RPE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Human recombinant ERp57 (Grp58) protein corresponding to aa 25-505 (his-tagged)
Specificity
Detects ~57 kDa protein.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDIA3 / ERp57
P30101 NM_005313 NP_005304.3

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/30/2024