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Catalog Number	Size	Price
LS-C796950-100	100 µg	$539

PDCD1 / CD279 / PD-1 Antibody - Sequential double staining of paraffin human melanoma using b-catenin(red) and PD1(brown). Both abs at 1:800 dilution of 1mg/mL; detection with Polink2 HRP DAB followed by Polink2 Broad AP. Anti-PD1: heat-induced epitope retrieval with Accel; anti-b-catenin: citrate pH6.0. Image shows tumor cells are strongly positve for b-catenin. (red) and negative for PD1. The activated T cells. (brown) show strong membranous and cytoplasmic staining for PD1 and no staining with b-catenin.

PDCD1 / CD279 / PD-1 Antibody - Sequential double staining of paraffin human lung using anti-b-Catenin(red) and anti-PD1(brown). Both antibodies at 1:800 dilution of 1mg/mL. Anti-PD1: heat-induced epitope retrieval with Accel; anti-b-Catenin: citrate pH6.0. Image shows tumor cells are strongly positve for b-catenin. (red) and negative for PD1. The arrows point to the activated T cells. (brown) showing strong membranous and cytoplasmic staining of PD1 and no staining with b-catenin.

PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of paraffin-embedded human colon cancer using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber. The image shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.

PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of paraffin-embedded human endometrial cancer using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber. The image shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.

PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 110C for 10 min. (1:1200)

PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of paraffin-embedded human prostate carcinoma using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber. The image shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.

PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of 3 paraffin-embedded human lung carcinomas using anti-PD1 clone UMAB199 mouse monoclonal antibody at 1:800 requires HIER Accel [OriGene/GBI Labs B22-125] in Pressure Chamber for 3 minute on high. Detection of primary antibody was achieved with Polink2 Broad HRP DAB [OriGene/GBI Labs D22].

PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of paraffin-embedded human melanoma using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber. The composite image of 3 melanoma shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.

PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber. The image shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.

PDCD1 / CD279 / PD-1 Antibody - Immunocytochemistry staining of PDCD1-stable-expression cells. (left) labeling PDCD1 with mouse anti-PDCD1 monoclonal antibody(1:900). The rihgt is negative control.

PDCD1 / CD279 / PD-1 Antibody - Immunofluorescent staining of PDCD1-stable-expression cells. (left) labeling PDCD1 with mouse monoclonal antibody(1:100, green) and nucleus with Hoechst33342. (blue). HEK293T cells serve as negative control. (right).

PDCD1 / CD279 / PD-1 Antibody - Western blot of human tissue lysates. (15ug) from 10 different tissues. (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.

PDCD1 / CD279 / PD-1 Antibody - Western blot of cell lysates. (35ug) from 9 different cell lines. (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500.

PDCD1 / CD279 / PD-1 Antibody - OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PDCD1 mouse monoclonal antibody. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. (1:100)

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Monoclonal Mouse anti‑Human PDCD1 / CD279 / PD‑1 Antibody (clone UMAB199, Carrier‑free, IHC, IF, WB) LS‑C796950

Name: Anti-PDCD1 / CD279 / PD-1 Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C796950-100
Price: 539.0000 USD
Availability: InStock

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Antibody:

PDCD1 / CD279 / PD-1 Mouse anti-Human Monoclonal (Carrier-free) (UMAB199) Antibody

Application:

IHC, IF, WB, PMA

Reactivity:

Human, Mouse, Rat, Dog

Format:

Unconjugated, Carrier-free

Other formats:

Unmodified

$539

LS-C796950-100

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

PDCD1 / CD279 / PD-1 Mouse anti-Human Monoclonal (Carrier-free) (UMAB199) Antibody

Application:

IHC, IF, WB, PMA

Reactivity:

Human, Mouse, Rat, Dog

Format:

Unconjugated, Carrier-free

Other formats:

Unmodified

Specifications

Description

PD-1 antibody LS-C796950 is an unconjugated mouse monoclonal antibody to PD-1 (PDCD1 / CD279) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, PMA and WB.

Target

Human PDCD1 / CD279 / PD-1

Synonyms

See All PDCD1 / CD279 / PD-1 Antibodies

Host

Mouse

Reactivity

Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)

Clonality

IgG2a Monoclonal

Clone

UMAB199

Conjugations

Unconjugated

Purification

Affinity chromatography

Modifications

Carrier-free. Also available Unmodified.

Immunogen

Full length human recombinant protein of human PDCD1 (NP_005009) produced in HEK293T cell.

Specificity

The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.

Applications

IHC (1:1000)
Immunofluorescence (1:100)
Western blot (1:500)
Protein Microarray

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

PBS, pH 7.3, 8% Trehalose

Reconstitution

Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About PDCD1 / CD279 / PD-1

Uniprot: Q15116 NCBI: NM_005018 NP_005009.2