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Catalog Number Size Price
LS-C796949-100 100 µg $539 
PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of paraffin-embedded human lung cancer using anti-PD-1 clone UMAB198 mouse monoclonal antibody  at 1:800 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Very strong cytoplasmic and membraneous staining in the activated Tcells no staining on the tumor.
PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 110C for 10 min. (1:1200)
PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of 3 cases of paraffin-embedded human tonsil using anti-PD-1 clone UMAB198 mouse monoclonal antibody  at 1:800 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Very strong cytoplasmic and membraneous staining in the activated Tcells of tonsil.
PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of paraffin-embedded human melanoma using anti-PD-1 clone UMAB198 mouse monoclonal antibody  at 1:800 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Very strong cytoplasmic and membraneous staining in the activated Tcells no staining on the tumor.
PDCD1 / CD279 / PD-1 Antibody - Immunocytochemistry staining of PDCD1-stable-expression cells. (left) labeling PDCD1 with mouse anti-PDCD1 monoclonal antibody(1:900). The rihgt is negative control.
PDCD1 / CD279 / PD-1 Antibody - Immunofluorescent staining of PDCD1-stable-expression cells. (left) labeling PDCD1 with mouse monoclonal antibody green) and nucleus with Hoechst33342. (blue). HEK293T cells serve as negative control. (right).
PDCD1 / CD279 / PD-1 Antibody - Western blot of human tissue lysates. (15ug) from 10 different tissues. (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.
PDCD1 / CD279 / PD-1 Antibody - Western blot of cell lysates. (35ug) from 9 different cell lines. (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500.
PDCD1 / CD279 / PD-1 Antibody - OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PDCD1 mouse monoclonal antibody. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. (1:100)
PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of paraffin-embedded human lung cancer using anti-PD-1 clone UMAB198 mouse monoclonal antibody  at 1:800 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Very strong cytoplasmic and membraneous staining in the activated Tcells no staining on the tumor.
PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 110C for 10 min. (1:1200)
PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of 3 cases of paraffin-embedded human tonsil using anti-PD-1 clone UMAB198 mouse monoclonal antibody  at 1:800 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Very strong cytoplasmic and membraneous staining in the activated Tcells of tonsil.
PDCD1 / CD279 / PD-1 Antibody - Immunohistochemical staining of paraffin-embedded human melanoma using anti-PD-1 clone UMAB198 mouse monoclonal antibody  at 1:800 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Very strong cytoplasmic and membraneous staining in the activated Tcells no staining on the tumor.
PDCD1 / CD279 / PD-1 Antibody - Immunocytochemistry staining of PDCD1-stable-expression cells. (left) labeling PDCD1 with mouse anti-PDCD1 monoclonal antibody(1:900). The rihgt is negative control.
PDCD1 / CD279 / PD-1 Antibody - Immunofluorescent staining of PDCD1-stable-expression cells. (left) labeling PDCD1 with mouse monoclonal antibody green) and nucleus with Hoechst33342. (blue). HEK293T cells serve as negative control. (right).
PDCD1 / CD279 / PD-1 Antibody - Western blot of human tissue lysates. (15ug) from 10 different tissues. (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.
PDCD1 / CD279 / PD-1 Antibody - Western blot of cell lysates. (35ug) from 9 different cell lines. (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500.
PDCD1 / CD279 / PD-1 Antibody - OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PDCD1 mouse monoclonal antibody. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. (1:100)
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Monoclonal Mouse anti‑Human PDCD1 / CD279 / PD‑1 Antibody (clone UMAB198, Carrier‑free, IHC, IF, WB) LS‑C796949

Monoclonal Mouse anti‑Human PDCD1 / CD279 / PD‑1 Antibody (clone UMAB198, Carrier‑free, IHC, IF, WB) LS‑C796949

Antibody:
PDCD1 / CD279 / PD-1 Mouse anti-Human Monoclonal (Carrier-free) (UMAB198) Antibody
Application:
IHC, IF, WB, PMA
Reactivity:
Human, Monkey, Mouse, Rat, Dog
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$539
LS-C796949-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PDCD1 / CD279 / PD-1 Mouse anti-Human Monoclonal (Carrier-free) (UMAB198) Antibody
Application:
IHC, IF, WB, PMA
Reactivity:
Human, Monkey, Mouse, Rat, Dog
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
PD-1 antibody LS-C796949 is an unconjugated mouse monoclonal antibody to PD-1 (PDCD1 / CD279) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, PMA and WB.
Target
Human PDCD1 / CD279 / PD-1
Synonyms
PDCD1 | CD279 | HPD-1 | HPD-l | PD1 | Protein PD-1 | SLEB2 | PD-1 | CD279 antigen | Programmed cell death 1
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
UMAB198
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Full length human recombinant protein of human PDCD1 (NP_005009) produced in HEK293T cell.
Specificity
The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.
Applications
  • IHC (1:100 - 1:1200)
  • Immunofluorescence (1:100)
  • Western blot (1:500)
  • Protein Microarray
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDCD1 / CD279 / PD-1
Q15116 NM_005018 NP_005009.2

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Requested From: United States
Date Requested: 4/30/2024