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Catalog Number Size Price
LS-C18957-100 100 µg (1.42 mg/ml) $498 
PCNA Antibody - Anti-Alga PCNA Antibody - Western Blot. Western blot of Affinity Purified anti-Alga PCNA antibody shows detection of a predominant band at ~29 kD corresponding to PCNA (arrowhead lane 2). Peptide competition effectively eliminates specific band reactivity (lane 1). For peptide competition, the antibody was pre-incubated with 100 ug (excess) of the peptide for 2h at 37? C followed by overnight incubation at 4C with 100 ug (excess) of the peptide, followed by a brief centrifugation and then reaction with the membrane. Each lane contains a lysate from 105 cells from the marine dinoflagellate Prorocentrum minimum. Cells were separated by SDS-PAGE and transferred onto nitrocellulose using standard techniques. After blocking the membrane was probed with the primary antibody diluted to 1:2001000. Incubation was followed by washes and reaction with HRP Gt-a-Rabbit IgG [H&L] MX (LS-C60865). Detection was by ECL using a 30 sec exposure time. Other detection systems will yield similar results. Personal Communication. S. Lin, U. Conn.

Polyclonal Rabbit anti‑Isochrysis galbana PCNA Antibody (WB) LS‑C18957

Polyclonal Rabbit anti‑Isochrysis galbana PCNA Antibody (WB) LS‑C18957

Antibody:
PCNA Rabbit anti-Isochrysis galbana Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Isochrysis galbana, Algae
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C18957-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PCNA Rabbit anti-Isochrysis galbana Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Isochrysis galbana, Algae
Format:
Unconjugated, Unmodified

Specifications

Description
PCNA antibody LS-C18957 is an unconjugated rabbit polyclonal antibody to PCNA from isochrysis galbana. It is reactive with algae and isochrysis galbana. Validated for ELISA, IP and WB.
Target
Isochrysis galbana PCNA
Synonyms
PCNA | Cyclin
Host
Rabbit
Reactivity
Isochrysis galbana, Algae (tested or 100% immunogen sequence identity)
Predicted
Algae (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 50-75 of Isochrysis galbana PCNA.
Specificity
A BLAST analysis was used to suggest that this antibody would react with PCNA from the following alga sources: Prorocentrum minimum, Tetraselmis chui, Pleurochrysis carterae and Isochrysis galbana. Expect partial reactivity with PCNA from other alga sources as well as carrot, tobacco, peas, soybean and rice based on partial protein sequence homology.  Species that have been tested but yielded no positive results are Pfiesteria piscicida, Emiliania huxleyi, Akashiwo sanguinea, Rhodomonas sp., Karlodinium micrum, and Skletonema costatum. Reactivity with PCNA from other sources has not been determined.
Applications
  • Western blot (1:5000 - 1:32000)
  • Immunoprecipitation (1:100)
  • ELISA (1:10000 - 1:50000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at -29 kD in size corresponding to PCNA by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PCNA
P12004 NM_002592 NP_002583.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022