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Catalog Number Size Price
LS-C742389-100 100 µg (0.5 mg/ml) $503 
PCNA Antibody - PCNA biotinylated antibody (0.05ug/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Polyclonal Goat anti‑Human PCNA Antibody (Biotin, aa111‑122, WB) LS‑C742389

Polyclonal Goat anti‑Human PCNA Antibody (Biotin, aa111‑122, WB) LS‑C742389

Antibody:
PCNA Goat anti-Human Polyclonal (aa111-122) (Biotin) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Biotin, Unmodified
Price
Catalog Number
$503
LS-C742389-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PCNA Goat anti-Human Polyclonal (aa111-122) (Biotin) Antibody
Application:
WB, Peptide-ELISA
Reactivity:
Human
Format:
Biotin, Unmodified

Specifications

Description
PCNA antibody LS-C742389 is a biotin-conjugated goat polyclonal antibody to human PCNA (aa111-122). Validated for Peptide-ELISA and WB.
Target
Human PCNA
Synonyms
PCNA | Cyclin
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Dog, Pig, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin
Purification
ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Modifications
Unmodified
Immunogen
EAPNQEKVSDYE
Epitope
aa111-122
Specificity
Reported variants represent identical protein: NP_872590.1, NP_002583.1
Applications
  • Western blot
  • Peptide Enzyme-Linked Immunosorbent Assay
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8kDa according to Human NP_002583.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gilljam et al, , J Cell Biol. 2009 Sep 7;186(5):645-54.). Recommended concentration: 0.03-0.1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PCNA
P12004 NM_002592 NP_002583.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/6/2024