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Catalog Number Size Price
LS-C745018-100 100 µg (1 mg/ml) $485 
PARP1 Antibody - Western Blot of recombinant PARP1 with Rabbit anti-PARP1 (internal) antibody. Lane 1: PARP1-autocatalytic domain recombinant protein. Load: 0.05 µg per lane. Primary antibody: PARP1 (internal) antibody at 1µg/mL for overnight at 4°C. Secondary antibody: HRP Gt-a-rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: ~19 kDa for rPARP1 (internal) Other band(s): none.
PARP1 Antibody - Western Blot of rabbit anti-PARP1 antibody. Lane 1: Molecular Weight ladder. Lane 2: OVCAR-8 Wild Type. Lane 3: PARP1-KO. Lane 4: PARP2-KO. Lane 5: PARP3-KO. Lane 6: PARP4-KO Lane 7: PARP5a-KO. Lane 8: PARP5b-KO. Lane 9: PARP6-KO. Lane 10: PARP7-KO. Lane 11: PARP8-KO. Lane 12: PARP9-KO. Lane 13: PARP10-KO. Lane 14: PARP12-KO. Lane 15: PARP13-KO. Lane 16: PARP14-KO. Lane 17: PARP16-KO. Load: 5.0 µg per lane. Primary antibody: PARP1 antibody at 1ug/mL overnight at 4°C. Secondary antibody: Goat anti-rabbit Peroxidase secondary antibody at 1:40,000 for 30 min at RT. Blocking Buffer: MB-073 for 30 min at RT. Predicted size: ~113kDa for PARP1. Observed nonspecific ~40kDa.
PARP1 Antibody - Western Blot of endogenous PARP1 with Rabbit Anti-PARP1 Antibodies. Lane 1: OVCAR8 Wild Type lysate. Lane 2: OVCAR8 PARP1 KO lysate. Load 5 ? per lane. Primary Antibody: Blot A: Anti-PARP1- n term; Blot B: Anti-PARP1- internal at 1?/mL for overnight at 4?. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4?. Predicted/Observed size: 113 kDa for endogenous PARP1. Other band(s): nonspecific ~ 40kDa in PARP1-AD only.
PARP1 Antibody - Western Blot of rabbit Anti-PARP1 (internal) Antibody. Lane 1: Wild Type control lysates. Lane 2: Knock Out control lysates. Load: 5 µg per lane. Primary antibody: PARP1 (internal) antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: ~113kDa endogenous for PARP1. Other band(s): nonspecific ~ 40kDa.
PARP1 Antibody - Peggy Sue Size Separation Electropherogram of OVCAR-8 lysates in no-salt buffer and detected with Anti-PARP1 (internal). UV immobilization time: 250 seconds. Protein concentration: 577 µg/mL; 120 s UV immobilization. Primary antibody concentration: 20µg/mL. Primary antibody incubation time: 180 min. Exposure time: 10 seconds. Predicted/observed: ~116 kDa.
PARP1 Antibody - OVCAR-8 Wild Type Lysate separated on 2D SDS-PAGE and blotted on PVDF to analyze immunocoverage of PARP1 antibody specific for the autocatalytic domain of PARP1. Primary Antibody: Anti-PARP1 (internal) antibody 1:200 overnight at 4°C. Secondary Antibody: Goat anti-rabbit Peroxidase at 1:2,000 at RT for 30min. Blocking Buffer: BlockOut for 30min at RT. Predicted/observed: ~110 kDa and pI 9.7. Other spots detected: cleavage products of PARP1.
PARP1 Antibody - Western Blot of recombinant PARP1 with Rabbit anti-PARP1 (internal) antibody. Lane 1: PARP1-autocatalytic domain recombinant protein. Load: 0.05 µg per lane. Primary antibody: PARP1 (internal) antibody at 1µg/mL for overnight at 4°C. Secondary antibody: HRP Gt-a-rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: ~19 kDa for rPARP1 (internal) Other band(s): none.
PARP1 Antibody - Western Blot of rabbit anti-PARP1 antibody. Lane 1: Molecular Weight ladder. Lane 2: OVCAR-8 Wild Type. Lane 3: PARP1-KO. Lane 4: PARP2-KO. Lane 5: PARP3-KO. Lane 6: PARP4-KO Lane 7: PARP5a-KO. Lane 8: PARP5b-KO. Lane 9: PARP6-KO. Lane 10: PARP7-KO. Lane 11: PARP8-KO. Lane 12: PARP9-KO. Lane 13: PARP10-KO. Lane 14: PARP12-KO. Lane 15: PARP13-KO. Lane 16: PARP14-KO. Lane 17: PARP16-KO. Load: 5.0 µg per lane. Primary antibody: PARP1 antibody at 1ug/mL overnight at 4°C. Secondary antibody: Goat anti-rabbit Peroxidase secondary antibody at 1:40,000 for 30 min at RT. Blocking Buffer: MB-073 for 30 min at RT. Predicted size: ~113kDa for PARP1. Observed nonspecific ~40kDa.
PARP1 Antibody - Western Blot of endogenous PARP1 with Rabbit Anti-PARP1 Antibodies. Lane 1: OVCAR8 Wild Type lysate. Lane 2: OVCAR8 PARP1 KO lysate. Load 5 ? per lane. Primary Antibody: Blot A: Anti-PARP1- n term; Blot B: Anti-PARP1- internal at 1?/mL for overnight at 4?. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4?. Predicted/Observed size: 113 kDa for endogenous PARP1. Other band(s): nonspecific ~ 40kDa in PARP1-AD only.
PARP1 Antibody - Western Blot of rabbit Anti-PARP1 (internal) Antibody. Lane 1: Wild Type control lysates. Lane 2: Knock Out control lysates. Load: 5 µg per lane. Primary antibody: PARP1 (internal) antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: ~113kDa endogenous for PARP1. Other band(s): nonspecific ~ 40kDa.
PARP1 Antibody - Peggy Sue Size Separation Electropherogram of OVCAR-8 lysates in no-salt buffer and detected with Anti-PARP1 (internal). UV immobilization time: 250 seconds. Protein concentration: 577 µg/mL; 120 s UV immobilization. Primary antibody concentration: 20µg/mL. Primary antibody incubation time: 180 min. Exposure time: 10 seconds. Predicted/observed: ~116 kDa.
PARP1 Antibody - OVCAR-8 Wild Type Lysate separated on 2D SDS-PAGE and blotted on PVDF to analyze immunocoverage of PARP1 antibody specific for the autocatalytic domain of PARP1. Primary Antibody: Anti-PARP1 (internal) antibody 1:200 overnight at 4°C. Secondary Antibody: Goat anti-rabbit Peroxidase at 1:2,000 at RT for 30min. Blocking Buffer: BlockOut for 30min at RT. Predicted/observed: ~110 kDa and pI 9.7. Other spots detected: cleavage products of PARP1.
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Polyclonal Rabbit anti‑Human PARP1 Antibody (C‑Terminus, WB) LS‑C745018

Polyclonal Rabbit anti‑Human PARP1 Antibody (C‑Terminus, WB) LS‑C745018

Antibody:
PARP1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, IE
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-C745018-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
PARP1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, IE
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PARP1 antibody LS-C745018 is an unconjugated rabbit polyclonal antibody to human PARP1 (C-Terminus). Validated for IE and WB.
Target
Human PARP1
Synonyms
PARP1 | ADPRT | ADP-ribosyltransferase NAD(+) | Adp-ribosyltransferase | ADPRT1 | ARTD1 | ADPRT 1 | Poly(ADP-ribose) synthetase | Poly(ADP-ribosyl)transferase | PARP | Poly [ADP-ribose] polymerase 1 | PADPRT-1 | PARP-1 | Poly (ADP-ribose) polymerase 1 | Poly(ADP-ribose) polymerase | Poly[ADP-ribose] synthase 1 | PPOL
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
PARP1 (internal) purified antibody was prepared from whole rabbit serum produced by repeated immunizations with c-terminus region of human PARP1 autocatalytic domain recombinant protein.
Epitope
C-Terminus
Specificity
This antibody is specific for human PARP1 protein. No cross reactivity detected towards other PARP members when using siRNAs against 18 PARP family members. Cross-reactivity with PARP1 from other sources has not been determined.
Applications
  • Western blot (1:1000)
  • Immunoelectrophoresis
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PARP1
P09874 NM_001618 NP_001609.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/12/2021