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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C2743-100 100 µl $335 
p19ARF (Mouse Cdkn2a) Antibody - Immunofluorescent detection of p19Arf in wild type mouse embryo fibroblasts (MEF) at passage 5 (lanes 1, 2) and NIH3T3 cells, which have deleted the Arf gene (lanes 3, 4) were fixed in methanol/acetone and probed with p19ARF, clone 12-A1-1 antibody, followed by a fluorescently labeled anti-rat IgG secondary antibody (lanes 1, 3). Cells were also stained with Hoechst dye to reveal the position of nuclei (lanes 2, 4).
p19ARF (Mouse Cdkn2a) Antibody - Western blot of endogenous p19Arf using p19ARF, clone 12-A1-1 antibody. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (lane 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (lane 2), were resolved by SDS-PAGE. The membrane was probed with 1u g/ml purified p19Arf (p19ARF, clone 12-A1-1 antibody.)
p19ARF (Mouse Cdkn2a) Antibody - Immunoprecipitation of p19Arf using p19ARF, clone 12-A1-1 antibody. Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (lane 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (lane 3), or p19ARF, clone 12-A1-1 antibody (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analyzed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) f.
p19ARF (Mouse Cdkn2a) Antibody - Immunofluorescent detection of p19Arf in wild type mouse embryo fibroblasts (MEF) at passage 5 (lanes 1, 2) and NIH3T3 cells, which have deleted the Arf gene (lanes 3, 4) were fixed in methanol/acetone and probed with p19ARF, clone 12-A1-1 antibody, followed by a fluorescently labeled anti-rat IgG secondary antibody (lanes 1, 3). Cells were also stained with Hoechst dye to reveal the position of nuclei (lanes 2, 4).
p19ARF (Mouse Cdkn2a) Antibody - Western blot of endogenous p19Arf using p19ARF, clone 12-A1-1 antibody. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (lane 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (lane 2), were resolved by SDS-PAGE. The membrane was probed with 1u g/ml purified p19Arf (p19ARF, clone 12-A1-1 antibody.)
p19ARF (Mouse Cdkn2a) Antibody - Immunoprecipitation of p19Arf using p19ARF, clone 12-A1-1 antibody. Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (lane 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (lane 3), or p19ARF, clone 12-A1-1 antibody (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analyzed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) f.
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p19ARF (Mouse Cdkn2a) Antibody (aa54‑75, clone 12A11) LS‑C2743

p19ARF (Mouse Cdkn2a) Antibody (aa54‑75, clone 12A11) LS‑C2743

Antibody:
Rat Monoclonal to Mouse p19ARF (Mouse Cdkn2a)
Application:
IF, WB, IP
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335 
LS-C2743-100
100 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rat Monoclonal to Mouse p19ARF (Mouse Cdkn2a)
Application:
IF, WB, IP
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
P19ARF (Mouse Cdkn2a) antibody LS-C2743 is an unconjugated rat monoclonal antibody to mouse p19ARF (Mouse Cdkn2a). Validated for IF, IP and WB.
Target
p19ARF (Mouse Cdkn2a)
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Non-Reactivity
Human, Hamster
Clonality
IgG2b Monoclonal
Clone
12A11
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (1:500)
  • Immunoprecipitation
Immunogen
A synthetic peptide containing amino acids within 1-100 of the murine p19ARF.
Epitope
aa54-75
Specificity
murine p19ARF. It does not recognize human p14Arf nor Golden hamster p13Arf.
Presentation
0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 11/18/2019