Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 7.5 µg/ml [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
PBS, pH 7.0, 0.01% Thimerosal, 20% Glycerol, 1% BSA
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.