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| Catalog Number | Size | Price |
|---|---|---|
| LS-C16928-100 | 100 µl | $634 |
Polyclonal Rabbit anti‑Human NOTCH1 Antibody (aa2487‑2501, IHC, WB) LS‑C16928
Polyclonal Rabbit anti‑Human NOTCH1 Antibody (aa2487‑2501, IHC, WB) LS‑C16928
Antibody:
NOTCH1 Rabbit anti-Human Polyclonal (aa2487-2501) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NOTCH1 Rabbit anti-Human Polyclonal (aa2487-2501) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
NOTCH1 antibody LS-C16928 is an unconjugated rabbit polyclonal antibody to human NOTCH1 (aa2487-2501). Validated for ELISA, IHC and WB. Cited in 2 publications.
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
A synthetic peptide C-QHSYSSPVDNTPSHQ corresponding to amino acid residues 2488-2502 of human Notch 1, intracellular domain. A residue of cysteine was added to the N-Terminus end to facilitate coupling. Percent identity by BLAST analysis: Human, Gorilla (100%); Opossum, Chicken, Xenopus, Newt (93%); Platypus (87%); Trout, Stickleback, Pufferfish, Zebrafish (80%).
Epitope
aa2487-2501
Specificity
Recognizes human NOTCH 1. No cross-reactivity with NOTCH 2 or Mouse Notch.
Applications
- IHC (1:500 - 1:2500)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in, ELISA, Immunohistochemistry and Western Blot. ELISA: 1:10000-1:50000. Standard sandwich ELISA using IgG, H&L (HRP) Pab Gt xRb and ABTS as a substrate for 30 minutes at RT. Western Blot: 1:1000-1:5000. Immunohistochemistry: 1:500-1:2500.
Presentation
PBS, 0.1% sodium azide. No stabilizing proteins added.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NOTCH1
LSBio Ratings
NOTCH1 Antibody (aa2487-2501) for IHC, WB/Western, ELISA LS-C16928 has an LSBio Rating of
Publications (4.1)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Sonic hedgehog (Shh)/Gli modulates the spatial organization of neuroepithelial cell proliferation in the developing chick optic tectum. Rapacioli M, Botelho J, Cerda G, Duarte S, Elliot M, Palma V, Flores V. BMC neuroscience. 2012 13:117. (IHC, WB; Human)
Temporal-spatial correlation between angiogenesis and corticogenesis in the developing chick optic tectum. Alejandra Rodriguez Celin, Melina Rapacioli, Mariela Azul Gonzalez, Virginia Laura Ballarin, Sara Fiszer de Plazas, Juan José López-Costa, Vladimir Flores. PloS one. 2015 January;10:e0116343.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
Date Requested: 4/27/2024
