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Catalog Number Size Price
LS-B398-50 50 µl (75 mg/ml) $495 
NOTCH1 Antibody - Anti-NOTCH1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

PathPlus™ Polyclonal Rabbit anti‑Human NOTCH1 Antibody (aa2480‑2510, IHC, WB) LS‑B398

PathPlus™ Polyclonal Rabbit anti‑Human NOTCH1 Antibody (aa2480‑2510, IHC, WB) LS‑B398

Note: This antibody replaces LS-C19108, LS-C73699
Antibody:
NOTCH1 Rabbit anti-Human Polyclonal (aa2480-2510) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$495
LS-B398-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
NOTCH1 Rabbit anti-Human Polyclonal (aa2480-2510) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NOTCH1 is a transmembrane receptor that binds Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. NOTCH1 affects the implementation of differentiation, proliferation and apoptotic programs. These include a number of functions in the brain, such as promoting progenitor cell differentiation into astrocytes and repressing neuronal and myogenic differentiation. NOTCH1 is also involved in angiogenesis, and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. During cerebellar development, NOTCH1 functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. In immunohistochemistry, NOTCH1 has positivity in most tissues throughout the body. References: Neuron. 29 (1): 45–55, PMID: 11182080; Neuron. 26 (2): 395–404, PMID: 10839358; Neuron. 26 (2): 395–404, PMID: 10839358;
Target
Human NOTCH1
Synonyms
NOTCH1 | Notch 1 | TAN1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
Anti-Notch antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid 2480-2510 of human Notch 1.  A residue of cysteine was added to the amino terminal end to facilitate coupling.
Epitope
aa2480-2510
Specificity
No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Other species have not been tested.
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B398 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B398 was determined to be 1:500.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NOTCH1
P46531 NM_017617 NP_060087.3

Validation

NOTCH1 Antibody - Anti-NOTCH1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-NOTCH1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-NOTCH1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

LSBio Ratings

PathPlus™ NOTCH1 Antibody (aa2480-2510) for IHC, WB/Western, ELISA LS-B398 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)

Publications (1)

Aberrant activation of notch signaling in human breast cancer. Stylianou S, Clarke RB, Brennan K. Cancer research. 2006 66:1517-25.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/23/2022