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Catalog Number Size Price
LS-C775532-100 100 µg (1 mg/ml) $445 
NO Tryptophan (Nitrotryptophan) Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 2D12. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody  at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Nitrotryptophan Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
NO Tryptophan (Nitrotryptophan) Antibody - Western Blot analysis of 6-Nitrotryptophan-BSA Conjugate showing detection of 67 kDa Nitrotryptophan protein using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 2D12. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (1 µg). Lane 4: 6-Nitrotryptophan-BSA (0.5 µg). Lane 5: 6-Nitrotryptophan-BSA (1 µg). Lane 6: 7-Ketocholesterol-BSA (0.5 µg). Lane 7: 7-Ketocholesterol-BSA (1 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: Luminol for 1 min. Predicted/Observed Size: 67 kDa.
NO Tryptophan (Nitrotryptophan) Antibody - Flow Cytometry analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 2D12. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody  at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
NO Tryptophan (Nitrotryptophan) Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 2D12. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody  at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Nitrotryptophan Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
NO Tryptophan (Nitrotryptophan) Antibody - Western Blot analysis of 6-Nitrotryptophan-BSA Conjugate showing detection of 67 kDa Nitrotryptophan protein using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 2D12. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (1 µg). Lane 4: 6-Nitrotryptophan-BSA (0.5 µg). Lane 5: 6-Nitrotryptophan-BSA (1 µg). Lane 6: 7-Ketocholesterol-BSA (0.5 µg). Lane 7: 7-Ketocholesterol-BSA (1 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: Luminol for 1 min. Predicted/Observed Size: 67 kDa.
NO Tryptophan (Nitrotryptophan) Antibody - Flow Cytometry analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 2D12. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody  at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
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NO Tryptophan (Nitrotryptophan) Antibody (clone 2D12, Atto 680) LS‑C775532

NO Tryptophan (Nitrotryptophan) Antibody (clone 2D12, Atto 680) LS‑C775532

Antibody:
Mouse Monoclonal to All species NO Tryptophan (Nitrotryptophan)
Application:
IF, WB, Flo, ELISA
Reactivity:
All species
Format:
Atto 680, Unmodified
Price
Catalog Number
$445 
LS-C775532-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to All species NO Tryptophan (Nitrotryptophan)
Application:
IF, WB, Flo, ELISA
Reactivity:
All species
Format:
Atto 680, Unmodified

Specifications

Description
NO Tryptophan (Nitrotryptophan) antibody LS-C775532 is an Atto 680-conjugated mouse monoclonal antibody to all species NO Tryptophan (Nitrotryptophan). Validated for ELISA, Flow, IF and WB.
Target
All species NO Tryptophan (Nitrotryptophan)
Host
Mouse
Reactivity
All species
Clonality
IgG1 Monoclonal
Clone
2D12
Conjugations
Atto 680
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:50)
  • ELISA (1:1000)
Immunogen
Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/17/2019