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Catalog Number Size Price
LS-C777295-100 100 µg (1 mg/ml) $445 
NO Tryptophan (Nitrotryptophan) Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 4F8. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody  at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Nitrotryptophan Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
NO Tryptophan (Nitrotryptophan) Antibody - Western Blot analysis of 6-Nitrotryptophan-BSA Conjugate showing detection of 67 kDa Nitrotryptophan protein using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 4F8. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (1 µg). Lane 4: 6-Nitrotryptophan-BSA (0.5 µg). Lane 5: 6-Nitrotryptophan-BSA (1 µg). Lane 6: 7-Ketocholesterol-BSA (0.5 µg). Lane 7: 7-Ketocholesterol-BSA (1 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: Luminol for 1 min. Predicted/Observed Size: 67 kDa.
NO Tryptophan (Nitrotryptophan) Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 4F8. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody  at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Nitrotryptophan Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
NO Tryptophan (Nitrotryptophan) Antibody - Western Blot analysis of 6-Nitrotryptophan-BSA Conjugate showing detection of 67 kDa Nitrotryptophan protein using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 4F8. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (1 µg). Lane 4: 6-Nitrotryptophan-BSA (0.5 µg). Lane 5: 6-Nitrotryptophan-BSA (1 µg). Lane 6: 7-Ketocholesterol-BSA (0.5 µg). Lane 7: 7-Ketocholesterol-BSA (1 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: Luminol for 1 min. Predicted/Observed Size: 67 kDa.
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NO Tryptophan (Nitrotryptophan) Antibody (clone 4F8, FITC) LS‑C777295

NO Tryptophan (Nitrotryptophan) Antibody (clone 4F8, FITC) LS‑C777295

Antibody:
Mouse Monoclonal to All species NO Tryptophan (Nitrotryptophan)
Application:
IF, WB, ELISA
Reactivity:
All species
Format:
FITC, Unmodified
Other formats:
Price
Catalog Number
$445 
LS-C777295-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to All species NO Tryptophan (Nitrotryptophan)
Application:
IF, WB, ELISA
Reactivity:
All species
Format:
FITC, Unmodified
Other formats:

Specifications

Description
NO Tryptophan (Nitrotryptophan) antibody LS-C777295 is an FITC-conjugated mouse monoclonal antibody to all species NO Tryptophan (Nitrotryptophan). Validated for ELISA, IF and WB.
Target
All species NO Tryptophan (Nitrotryptophan)
Host
Mouse
Reactivity
All species
Clonality
IgG2b Monoclonal
Clone
4F8
Conjugations
FITC. Also available conjugated with AP, APC, Atto 390, Atto 488, Atto 565, Atto 594, Atto 633, Atto 655, Atto 680, Atto 700, Biotin, HRP, PE, Atto 594, PerCP, RPE, SA.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:50)
  • Western blot (1:1000)
  • ELISA (1:1000)
  • Applications tested for the base form of this product only
Immunogen
Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/13/2019