Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
R. Phycoerythrin.
Also available conjugated with
FITC.
Purification
Protein G purified
Immunogen
CHO transfected cells expressing the B6 allele of NKG2A.
Specificity
Recognizes murine NKG2A, NKG2C and NKG2E, which are isoforms of the NKG2 lectin-like family. In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells. The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse. Clone 20d5 is reported to block ligand binding to the receptor(1). XXX recommend the use of MCA2287EL for this purpose.
Applications
- Flow Cytometry (1:1 - 1:2)
- Applications tested for the base form of this product only
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. beforeStore undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee