Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C776857-100 100 µg (1 mg/ml) $442 
NIP3 / BNIP3 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BNIP3 Polyclonal Antibody. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BNIP3 Antibody (D) Composite.
NIP3 / BNIP3 Antibody - Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa.
NIP3 / BNIP3 Antibody - Western blot analysis of Mouse Kidney showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa. Other Band(s): ~60kDa (BNIP3 complex).
NIP3 / BNIP3 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BNIP3 Polyclonal Antibody. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BNIP3 Antibody (D) Composite.
NIP3 / BNIP3 Antibody - Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa.
NIP3 / BNIP3 Antibody - Western blot analysis of Mouse Kidney showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa. Other Band(s): ~60kDa (BNIP3 complex).
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human NIP3 / BNIP3 Antibody (IF, WB) LS‑C776857

Polyclonal Rabbit anti‑Human NIP3 / BNIP3 Antibody (IF, WB) LS‑C776857

Antibody:
NIP3 / BNIP3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$442
LS-C776857-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NIP3 / BNIP3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
BNIP3 antibody LS-C776857 is an unconjugated rabbit polyclonal antibody to BNIP3 (NIP3) from human. It is reactive with human and mouse. Validated for IF and WB.
Target
Human NIP3 / BNIP3
Synonyms
BNIP3 | NIP3
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the C-Terminus of human BNIP3
Specificity
Predicted molecular weight at ~21.5 kDa. Observed bands at ~20-30 kDa and 50-60 kDa due to the homodimer complex of BNIP3.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NIP3 / BNIP3
Q12983 NM_004052 NP_004043.3

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024