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Antibodies > NIP3 / BNIP3 > LS-C98865
 
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Catalog Number Size Price
LS-C98865-400 400 µl $393 
NIP3 / BNIP3 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
NIP3 / BNIP3 Antibody - Freshly isolated mouse hepatocytes plated on coverslips (2 x105 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with anti-BNIP3 antibody. Data courtesy of Ruben Zamora, University of Pittsburgh.
NIP3 / BNIP3 Antibody - Fluorescent confocal image of HepG2 cells stained with BNIP3 (BH3 Domain Specific) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated BNIP3 (BH3 Domain Specific) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.
NIP3 / BNIP3 Antibody - The anti-NIP3 BH3 domain antibody is used in Western blot to detect NIP3 BH3 in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2).
NIP3 / BNIP3 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
NIP3 / BNIP3 Antibody - Freshly isolated mouse hepatocytes plated on coverslips (2 x105 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with anti-BNIP3 antibody. Data courtesy of Ruben Zamora, University of Pittsburgh.
NIP3 / BNIP3 Antibody - Fluorescent confocal image of HepG2 cells stained with BNIP3 (BH3 Domain Specific) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated BNIP3 (BH3 Domain Specific) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.
NIP3 / BNIP3 Antibody - The anti-NIP3 BH3 domain antibody is used in Western blot to detect NIP3 BH3 in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2).
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Polyclonal Rabbit anti‑Human NIP3 / BNIP3 Antibody (aa152‑187, IHC, IF, WB) LS‑C98865

Polyclonal Rabbit anti‑Human NIP3 / BNIP3 Antibody (aa152‑187, IHC, IF, WB) LS‑C98865

(4.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Antibody:
NIP3 / BNIP3 Rabbit anti-Human Polyclonal (aa152-187) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C98865-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NIP3 / BNIP3 Rabbit anti-Human Polyclonal (aa152-187) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
BNIP3 antibody LS-C98865 is an unconjugated rabbit polyclonal antibody to BNIP3 (NIP3) (aa152-187) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Cited in 1 publication.
(1) Publication(s)
Target
Human NIP3 / BNIP3
Synonyms
BNIP3 | NIP3
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa152-187
Specificity
This BNIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-187 amino acids from human BNIP3.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NIP3 / BNIP3
Q12983 NM_004052 NP_004043.3

LSBio Ratings

NIP3 / BNIP3 Antibody (aa152-187) for IHC, IF/Immunofluorescence, WB/Western LS-C98865 has an LSBio Rating of
(4.0) Rating
Publications (4)

Publications (1)

Chronic autophagy is a cellular adaptation to tumor acidic pH microenvironments. Wojtkowiak JW, Rothberg JM, Kumar V, Schramm KJ, Haller E, Proemsey JB, Lloyd MC, Sloane BF, Gillies RJ. Cancer research. 2012 72:3938-47.
PubMed: 22719070
Submit a Publication for LS-C98865

Customer Reviews (0)

Submit a review for LS-C98865

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NIP3 / BNIP3 Antibody - This image was taken for the unconjugated form of this product. Other forms have not been tested.
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NIP3 / BNIP3 Antibody - Immunohistochemistry: BNIP3 Antibody - Analysis of BNIP3 in mouse kidney using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Species: Human, Mouse
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Mouse NIP3 / BNIP3 Protein (Recombinant 10His, N-terminus) (Full Length) - LS-G136896
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Tag: 10His, N-terminus
NIP3 / BNIP3 Protein
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Source: E. coli
Tag: His, N-Terminal
See all NIP3 / BNIP3 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
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