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Catalog Number Size Price
LS-C408085-10 10 µg $318 
LS-C408085-100 100 µg $470 
NES / Nestin Antibody - IHC analysis of Nestin using anti-Nestin antibody. Nestin was detected in frozen section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Nestin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NES / Nestin Antibody - IHC analysis of Nestin using anti-Nestin antibody. Nestin was detected in frozen section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Nestin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NES / Nestin Antibody - Nestin was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- Nestin Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
NES / Nestin Antibody - Nestin was detected in paraffin-embedded sections of human melanoma tissues using rabbit anti- Nestin Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
NES / Nestin Antibody - Nestin was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti-Nestin Antigen Affinity purified polyclonal antibody at 1 ug/ml. The immunohistochemical section was developed using SABC method
NES / Nestin Antibody - Western blot analysis of Nestin expression in HELA whole cell lysates (lane 1) and HEPG2 whole cell lysates (lane 2). Nestin at 230 kD was detected using rabbit anti- Nestin Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
NES / Nestin Antibody - IHC analysis of Nestin using anti-Nestin antibody. Nestin was detected in frozen section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Nestin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NES / Nestin Antibody - IHC analysis of Nestin using anti-Nestin antibody. Nestin was detected in frozen section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Nestin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NES / Nestin Antibody - Nestin was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- Nestin Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
NES / Nestin Antibody - Nestin was detected in paraffin-embedded sections of human melanoma tissues using rabbit anti- Nestin Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
NES / Nestin Antibody - Nestin was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti-Nestin Antigen Affinity purified polyclonal antibody at 1 ug/ml. The immunohistochemical section was developed using SABC method
NES / Nestin Antibody - Western blot analysis of Nestin expression in HELA whole cell lysates (lane 1) and HEPG2 whole cell lysates (lane 2). Nestin at 230 kD was detected using rabbit anti- Nestin Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
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Polyclonal Rabbit anti‑Human NES / Nestin Antibody (aa252‑290, IHC, WB) LS‑C408085

Polyclonal Rabbit anti‑Human NES / Nestin Antibody (aa252‑290, IHC, WB) LS‑C408085

Antibody:
NES / Nestin Rabbit anti-Human Polyclonal (aa252-290) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat, Horse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C408085-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NES / Nestin Rabbit anti-Human Polyclonal (aa252-290) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat, Horse
Format:
Unconjugated, Unmodified

Specifications

Description
Nestin antibody LS-C408085 is an unconjugated rabbit polyclonal antibody to Nestin (NES) (aa252-290) from human. It is reactive with human, rat and horse. Validated for IHC and WB.
Target
Human NES / Nestin
Synonyms
NES | Nestin | Nbla00170
Host
Rabbit
Reactivity
Human, Rat, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human Nestin (252-290 aa ERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAH), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.
Epitope
aa252-290
Specificity
CNS stem cells.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NES / Nestin
P48681 X65964 CAA46780.1

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Requested From: United States
Date Requested: 4/30/2024