Visfatin antibody LS-B12879 is an unconjugated mouse monoclonal antibody to Visfatin (NAMPT) from human, mouse and rat. Validated for ELISA, Flow, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Human NAMPT / Visfatin
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Protein G purified from tissue culture supernatant
- IHC - Paraffin (1:500 - 1:1000) & (15 µg/ml)
- ICC (1:500 - 1:1000)
- Western blot (1:2000 - 1:5000)
- Immunoprecipitation (1:500 - 1:1000)
- Flow Cytometry (1:1000)
- ELISA (1:2000 - 1:10000)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Recombinant human Nampt (visfatin/PBEF).
Recognizes human, mouse and rat Nampt.
ELISA - Direct or indirect: 1:2000-1:10000. Flow Cytometry - 1:1000. Immunocytochemistry - 1:500-1:1000. Immunohistochemistry - Paraffin sections: 15ug/ml. Immunoprecipitation - 1:500-1:1000. Western blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.
PBS, pH 7.4, no preservatives.
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.