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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NAMPT / Visfatin Antibody (clone OMNI379) IHC-plus™ LS-B12879

Note: This antibody replaces LS-C344877
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).
Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.
Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.
Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).
Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.
Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.
Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.
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NAMPT / Visfatin Antibody (clone OMNI379) IHC-plus™ LS-B12879

Note: This antibody replaces LS-C344877
Antibody:
Mouse Monoclonal to Human NAMPT / Visfatin
Application:
IHC, IHC-P, ICC, IF, WB, IP, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B12879-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human NAMPT / Visfatin
Application:
IHC, IHC-P, ICC, IF, WB, IP, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Visfatin antibody LS-B12879 is an unconjugated mouse monoclonal antibody to Visfatin (NAMPT) from human, mouse and rat. Validated for ELISA, Flow, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human NAMPT / Visfatin
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
OMNI379
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500 - 1:1000) & (15 µg/ml)
  • ICC (1:500 - 1:1000)
  • Immunofluorescence
  • Western blot (1:2000 - 1:5000)
  • Immunoprecipitation (1:500 - 1:1000)
  • Flow Cytometry (1:1000)
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human Nampt (visfatin/PBEF).
Specificity
Recognizes human, mouse and rat Nampt.
Usage
ELISA - Direct or indirect: 1:2000-1:10000. Flow Cytometry - 1:1000. Immunocytochemistry - 1:500-1:1000. Immunohistochemistry - Paraffin sections: 15ug/ml. Immunoprecipitation - 1:500-1:1000. Western blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NAMPT / Visfatin
P43490 NM_005746 NP_005737.1

Validation

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Validated
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

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Requested From: United States
Date Requested: 6/17/2019
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