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Antibodies > NAMPT / Visfatin

NAMPT / Visfatin Antibody (clone OMNI379) IHC-plus™ LS-B12879

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Note: This antibody replaces LS-C344877
Visfatin antibody LS-B12879 is an unconjugated mouse monoclonal antibody to Visfatin (NAMPT) from human, mouse and rat. Validated for ELISA, Flow, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
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Size
Price
LS-B12879-50
50 µg (1 mg/ml)
$425
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Description
Visfatin antibody LS-B12879 is an unconjugated mouse monoclonal antibody to Visfatin (NAMPT) from human, mouse and rat. Validated for ELISA, Flow, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NAMPT / Visfatin
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
OMNI379
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500 - 1:1000) & (15 µg/ml)
  • ICC (1:500 - 1:1000)
  • Immunofluorescence
  • Western blot (1:2000 - 1:5000)
  • Immunoprecipitation (1:500 - 1:1000)
  • Flow Cytometry (1:1000)
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human Nampt (visfatin/PBEF).
Specificity
Recognizes human, mouse and rat Nampt.
Usage
ELISA - Direct or indirect: 1:2000-1:10000. Flow Cytometry - 1:1000. Immunocytochemistry - 1:500-1:1000. Immunohistochemistry - Paraffin sections: 15ug/ml. Immunoprecipitation - 1:500-1:1000. Western blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
About NAMPT / Visfatin
P43490 NM_005746 NP_005737.1

Popular NAMPT / Visfatin Products

Western blot of NAMPT / Visfatin antibody.
NAMPT / Visfatin Antibody LS‑C48964
Species: Mouse, Human
Applications: Western blot
Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
NAMPT / Visfatin Antibody (aa1‑491, clone 4D5) LS‑C93818
Species: Human
Applications: Western blot, ELISA
Western blot of NAMPT / Visfatin antibody.
NAMPT / Visfatin Antibody LS‑C48965
Species: Rat
Applications: Western blot
NAMPT antibody ARP42255_T100-NP_005737-PBEF1(pre-B-cell colony enhancing factor 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NAMPT / Visfatin Antibody (C‑Terminus) LS‑C31889
Species: Human, Guinea pig, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Western blot of NAMPT in human tumor cell lines at 1:1000. Lanes: A. HeLa, B. Jurkat, C. Daudi, D. HEK 293, E. Rh30, F. A375, G. T98G, H. HCT-116, I. Hep-2.
NAMPT / Visfatin Antibody LS‑C47455
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Chicken, Xenopus, Zebrafish
Applications: Western blot, ELISA
More NAMPT / Visfatin Products

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Images

Immunohistochemistry - Paraffin

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).
Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).

Western blot

Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.
Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.

Western blot

Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.
Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.

Western blot

Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.
Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.

Immunohistochemistry - Paraffin

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).
Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).

Western blot

Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.
Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.

Western blot

Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.
Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.

Western blot

Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.
Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.

Immunohistochemistry - Paraffin

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).
Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).

Western blot

Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.
Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.

Western blot

Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.
Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.

Western blot

Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.
Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.

Immunohistochemistry - Paraffin

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).
Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).

Western blot

Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.
Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2,000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.

Western blot

Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.
Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.

Western blot

Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.
Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2,000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.
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Requested From: United States
Date Requested: 2/19/2019
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