MTOR antibody LS-C16625 is an unconjugated rabbit polyclonal antibody to MTOR from human, mouse, rat and other species. Validated for ELISA, IP and WB.
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
- Western blot (1:1000)
- Immunoprecipitation (1:100)
MTOR antibody was raised against synthetic peptide (KLH coupled) corresponding to residues surrounding Ser2481 of human mTOR.
Detects endogenous levels of total human mTOR. It cross-reacts weakly with some other proteins based on Western analysis. Species cross-reactivity: Mouse, monkey and rat.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:100.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.