Target
Human MTOR
Synonyms
MTOR | FRAP1 | FRAP2 | FRAP | Mammalian target of rapamycin | RAFT1 | Rapamycin and FKBP12 target 1 | RAPT1 | Rapamycin target protein 1
Reactivity
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide (KLH coupled) corresponding to residues surrounding Ser2481 of human mTOR.
Specificity
Detects endogenous levels of total human mTOR. It cross-reacts weakly with some other proteins based on Western analysis. Species cross-reactivity: Mouse, monkey and rat.
Applications
- Western blot (1:1000)
- Immunoprecipitation (1:100)
- ELISA
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:100.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee