Target
Human MTOR
Synonyms
MTOR | FRAP1 | FRAP2 | FRAP | Mammalian target of rapamycin | RAFT1 | Rapamycin and FKBP12 target 1 | RAPT1 | Rapamycin target protein 1
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser2448 of human mTOR.
Specificity
Detects endogenous levels of human mTOR only when phosphorylated at Ser2448. Species cross-reactivity: Mouse and rat.
Applications
- Western blot (1:1000)
- Immunoprecipitation (1:100)
- ELISA
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:100.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee