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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MRE11A / MRE11 Antibody (aa578-590) LS-C18848

Anti-Mre11 (S. cerevisiae) Antibody - Chromatin Immunoprecipitation (ChIP). Chromatin Immunoprecipitation (ChIP) using Affinity Purified Mre11 (S. cerevisiae) antibody. A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. immune complexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR Green (Invitrogen), and detected using a Fuji scanning fluorimeter. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.

MRE11A / MRE11 Antibody (aa578-590) LS-C18848

Antibody:
Rabbit Polyclonal to Yeast MRE11A / MRE11
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C18848-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Yeast MRE11A / MRE11
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified

Specifications

Description
MRE11 antibody LS-C18848 is an unconjugated rabbit polyclonal antibody to yeast MRE11 (MRE11A). Validated for ELISA and WB.

Target
Yeast MRE11A / MRE11
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:200 - 1:2000)
  • ELISA (1:10000 - 1:50000)
Immunogen
MRE11A / MRE11 antibody was raised against synthetic peptide corresponding to amino acids 578-590 of Saccharomyces cerevisiae (baker's yeast) Mre11.
Epitope
aa578-590
Specificity
Amino acids 578-590 of Saccharomyces cerevisiae (baker's yeast) Mre11 protein.
Usage
This affinity purified antibody has been tested for use in ELISA and by ChIP assay. Specific conditions for reactivity should be optimized by the end user.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MRE11A / MRE11
P49959 NM_005591 NP_005582.1

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Reactivity: Human
Range: 31.25-2000 pg/ml
Reactivity: Human
Range: 15.6-1000 pg/ml
Reactivity: Mouse
Range: 15.63-1000 pg/ml
Reactivity: Mouse
Range: 15.63-1000 pg/ml

Requested From: United States
Date Requested: 7/19/2019
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