Human Lambda Light Chain

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

NI412, NI 268

Unconjugated. Also available conjugated with Biotin, HRP.

Ascites

Unmodified

Highly purified Bence Jones lambda proteins isolated from pooled human urine.

Human Ig lambda chain, free and bound.

• Immunofluorescence
• ELISA

The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. in ELISA from 1:200 upwards. in Western blotting from 1:400 upwards. These data should be interpreted as general recommendations only.

Lyophilized No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.