Target
Human Lambda Light Chain
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Horseradish Peroxidase.
Also available
Unconjugated or conjugated with
Biotin.
Purification
Purified IgG
Immunogen
Highly purified Bence Jones lambda proteins isolated from pooled human urine.
Specificity
Human Ig lambda chain, free and bound.
Applications
- IHC
- IHC - Paraffin
- ICC
- Immunofluorescence
- ELISA
- Dot Blot
- Applications tested for the base form of this product only
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Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. in ELISA from 1:50 upwards. in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
Reconstitute with distilled water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee