Human Kappa Light Chain

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

NI 285, NI 250

Tetramethyl Rhodamine Iso-Thiocyanate. Also available Unconjugated or conjugated with Biotin, FITC, HRP.

Purified IgG

Unmodified

Highly purified Bence Jones kappa proteins isolated from pooled human urine.

Human Ig kappa chain, free and bound.

• IHC
• IHC - Frozen
• ICC
• Immunofluorescence
• ELISA
• Applications tested for the base form of this product only

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining and ELISA and immunoblotting using an indirect technique with monoclonal anti TRITC labeled with peroxidase. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:200. in ELISA from 1:500 upwards. in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.