Human Kappa Light Chain

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

NI 285, NI 250

Biotin. Also available Unconjugated or conjugated with FITC, HRP, TRITC.

Purified

Unmodified

Highly purified Bence Jones kappa proteins isolated from pooled human urine.

Human Ig kappa chain, free and bound.

• IHC
• IHC - Paraffin
• ICC
• Immunofluorescence
• ELISA
• Dot Blot
• Applications tested for the base form of this product only

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of this preparation of two monoclonal antibodies is restricted to a surface determinant on the kappa light chain. It is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500. in ELISA from 1:1000 upwards. in Western blotting from 1:2.000 upwards. These data should be interpreted as general recommendations only.

No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.